conclusions our findings revealed that the zfn technology could be employed to overcome ampicillin resistance by the targeted disruption of the ampicillin resistance gene, which leads to inactivation of β-lactam synthesis. therefore, zfn technology could be engaged to decrease the antibiotic resistance issue with the construction of a zfn archive against different args. to tackle the resistance...

conclusion although all esbl producer gram-negative bacteria were sensitive to imipenem, piperacillin-tazobactam, and colistin, non-esbl isolates showed resistant pattern. interestingly, notable percent of mentioned resistant isolates were sensitive to nitrofurantoin. background resistance in enteric gram-negative bacteria is of a great concern and concise local data are lacking. objectives to ...

background : uncontrolled and irrational use of antibiotics increases the rate of antimicrobial resistance and treatment failure. compliance with antibiotics is an important indicator to show how patients use their prescribed drugs and it can explain the relationship between drug administration and treatment outcome that needs to be monitored and promoted. we decided to evaluate compliance to a...

conclusions the results of the antibiotic resistance and other data suggest that there is a possible common clone of e. faecalis, which could rapidly disseminate in patients and the environment. results the results indicated that, 86% of patient and 29% of environmental isolates carried ebp genes. the ability of the isolates to strongly attach was 62% and 71% for patient and environmental sampl...

The emergence and spread of antibiotic resistance is a crisis in health care today. Antibiotic resistance is often horizontally transferred to susceptible bacteria by means of multidrug resistance plasmids that may or may not persist in the absence of antibiotics. Because bacterial pathogens often grow as biofilms, there is a need to better understand the evolution of plasmid persistence in the...

Results Studies show that bacterial resistance is increasing in parallel with antibiotic use, it is estimated by the World Health Organization (WHO) that 25 to 33% of patients use antimicrobial drugs during their hospitalization. Although the choice of antibiotic is a doctor’s concern , the administration of drugs is carried out by nursing staff who are nursing assistants and technicians being ...

Globally, pneumonia is considered to be the biggest killer of infants and young children (aged <5 years) outside the neonatal period, with the greatest disease burden in low- and middle-income countries. Optimal management of childhood pneumonia is challenging in settings where clinicians have limited information regarding the local pathogen and drug resistance profiles. This frequently results...

Biofilms are difficult to eliminate with standard antimicrobial treatments due to their high antibiotic resistance relative to free-living cells. Here, we show that selected antimicrobial essential oils can eradicate bacteria within biofilms with higher efficiency than certain important antibiotics, making them interesting candidates for the treatment of biofilms.

This study focused on the presence of antibiotic-resistant bacteria in a metro system as an example of a public transportation system. The molecular characteristics of Staphylococcus were investigated to discern which strains were isolated from metro stations in Shanghai. These were compared with strains isolated from hospital treatment rooms and parks. Airborne Staphylococcus samples in the me...