The DRB3 gene is a highly polymorphic major histocompatibility complex (MHC) class II gene and plays an important role in variability of immune responsiveness and disease resistance. In the present study, the MHC class II DRB3 gene in water buffalo (Bubalus bubalis) populations from Northwest regions of Iran was investigated through PCR-SSCP. Genomic DNA was extracted from whole blood samples c...

MHC class II tetramers have emerged as an important tool for characterization of the specificity and phenotype of CD4 T cell immune responses, useful in a large variety of disease and vaccine studies. Issues of specific T cell frequency, biodistribution, and avidity, coupled with the large genetic diversity of potential class II restriction elements, require targeted experimental design. Transl...

We screened natural chemical compounds which inhibit the expression of newly-synthesized MHC class II molecules on the cell surface. IFN-gamma stimulates Colo 205 adenocarcinoma cells to induce the expression of MHC class I, MHC class II, and ICAM-1 molecules on the cell surface. We show here that concanamycin B inhibits the expression of MHC class II molecules on Colo 205 cells, whereas it doe...

The intracellular mechanism by which interferon-gamma induces the expression of class II major histocompatibility complex (MHC) antigen in nonlymphoid cells is not clear. The effect of recombinant rat interferon-gamma (IFN-gamma), and cycloheximide on the expression of class II MHC gene was studied using the techniques of immunocytochemical staining and northern blot analysis. IFN-gamma induced...

the drb3 gene is a highly polymorphic major histocompatibility complex (mhc) class ii gene and plays an important role in variability of immune responsiveness and disease resistance. in the present study, the mhc class ii drb3 gene in water buffalo (bubalus bubalis) populations from northwest regions of iran was investigated through pcr-sscp. genomic dna was extracted from whole blood samples c...