THE BASIS FOR EDTA-STIMULATION OF METHEMOGLOBIN REDUCTION IN HEMOLYSATES OF HUMAN ERYTHROCYTES Lucy Jean Sannes* and Donald E. Hultquist ** Department of Biological Chemistry The University of Michigan Ann Arbor, Michigan 48109 Received November lo,1979 Summary: We have studied the stimulation by EDTA of methemoglobin reduction in hemolysates of human erythrocytes. The EDTA effect has been show...

Interspecies variability of propylene glycol dinitrate (PGDN)-induced methemoglobin formation was studied in vitro employing erythrocytes from four separate species. The net rate of methemoglobin formation was significantly different among species with dog greater than guinea pig greater than rat greater than or equal to human. This order of susceptibility was maintained in stroma-free hemolysa...

background: malaria remains the world’s most devastating human parasitic infection. our goal was to assess the capacity of increasing concentrations of five antimalarial drugs (fansidartm, halfantm, quinine, coartemtm and chloroquine phosphate) to elicit the generation of methemoglobin in three human erythrocyte genotypes (hb aa, hb as and hb ss). materials and methods: spectrophotometric metho...

A major concern during pesticide development and use is the impact on non-target species, such as raptors or domestic cats and dogs. Sodium nitrite and para-aminopropiophenone (PAPP) are two toxicants currently being studied for the control of invasive species, such as starlings and feral swine. When given to an animal these compounds oxidize hemoglobin, which renders it unable to carry oxygen ...

Severe intravascular hemolysis leads to the simultaneous presence of free heme pigments (oxyhemoglobin, methemoglobin, and methemalbumin) bilirubin in human plasma. Standard spectrophotometric methods used assess vivo inadequately address this complex analytical situation. Thus, we propose a novel quantification algorithm ensure highest specificity. A corresponding second-derivative fitting was...

The rate of methemoglobin reduction by ascorbic acid was accelerated in the presence of ATP,2,3-diphosphoglycerate (2,3-DPG), and inositol hexaphosphate (IHP). The acceleration was as much as three times, four times, and ten times in the presence of ATP, 2.3-DPG, and IHP at pH 7.0, respectively. The changes of the concentrations of methemoglobin and ascorbic acid during the methemoglobin reduct...

cyanosis develops in the absence of any car. diac or respiratory abnormalities.1 Cyanosis is a result of the accumulation of methemoglobin, which imparts a bluish color to the blood. This condition has two forms: a hereditary and an acquired form. Hereditary methemoglobinemia is an extremely rare inborn error of metabolism resulting in the accumulation of methemoglobin. 2 The acquired form resu...

BACKGROUND Methemoglobin in the blood cannot be detected by conventional pulse oximetry and may bias the oximeter's estimate (Spo(2)) of the true arterial functional oxygen saturation (Sao(2)). A recently introduced "pulse CO-oximeter" (Masimo Rainbow SET® Radical-7) that measures SpMet, a noninvasive measurement of the percentage of methemoglobin in arterial blood (%MetHb), was shown to read s...

INTRODUCTION Surgery is supposed to modulate the production of carbon monoxide by the reduction of heme oxygenase activity or transcriptional regulation of inducible heme oxygenase. On the other hand, the inhalation of tobacco smoke can substantially raise the level of carboxyhemoglobin in the blood. Furthermore, methemoglobin is maintained at a constant level. However, excessive production of ...