BACKGROUND Pichia pastoris is an established eukaryotic host for the production of recombinant proteins. Most often, protein production is under the control of the strong methanol-inducible aox1 promoter. However, detailed information about the physiological alterations in P. pastoris accompanying the shift from growth on glycerol to methanol-induced protein production under industrial relevant...

A novel route is proposed for the preparation of mesopore containing zeolite ZSM-5 via in situ hydrothermal treatment of a solution containing alkali-dissolved SBA-15 containing carbonized surfactant P123 in the mesopores; it exhibited prominent stability enhancement for methanol to propylene reaction.

Fluorescence spectra of anti-tumoral drug emodin loaded on nanostructured porous silicon have been recorded. The use of colloidal nanoparticles allowed embedding of the drug without previous porous silicon functionalization and leads to the observation of an enhancement of fluorescence of the drug. Mean pore size of porous silicon matrices was 60 nm, while silver nanoparticles mean diameter was...

Recombinant elastase strain K overexpressed from E. coli KRX/pCon2(3) was purified to homogeneity by a combination of hydrophobic interaction chromatography and ion exchange chromatography, with a final yield of 48% and a 25-fold increase in specific activity. The purified protein had exhibited a first ever reported homodimer size of 65 kDa by SDS-PAGE and MALDI-TOF, a size which is totally dis...

In the first part of two section paper, a mathematical model of the fluidized bed reactor in the presence of in-situ water adsorbent for methanol synthesis is assessed. The bubbling two-phase regime is applied to model the fluidization concept. The binary adsorbent and catalyst particles system can be separated from each other based on their density difference. The heavy catalyst particles tend...

Nowadays, hydrogen and methanol are attractive prospects because of lower emission compared to the other energy sources and their special application in fuel cell technology, which are now widely regarded as key energy solution for the 21st century. These two chemicals also can be utilized in transportation, distributed heat and power generation and energy storage systems. In this study, a nove...

poly-β-hydroxybutyrate (phb), produced by several species of bacteria, has attracted great attention as a biodegradable and biocompatible compound with almost similar properties of polypropylene. unfortunately, its use is currently limited due to high production costs. one of the most common methods for overcoming this constraint is the use of inexpensive substrates, like methanol. another stra...

Dimethyl ether (DME) has been considered as a promising alternative fuel for direct-feed fuel cells but lack of an efficient DME oxidation electrocatalyst has remained the challenge for the commercialization of the direct DME fuel cell. The commonly studied binary PtRu catalyst shows much lower activity in DME than methanol oxidation. In this work, guided by density functional theory (DFT) calc...

Monodispersed bimetallic PdAg nanoparticles can be fabricated through the emulsion-assisted ethylene glycol (EG) ternary system. Different compositions of bimetallic PdAg nanoparticles, Pd₈₀Ag₂₀, Pd₆₅Ag₃₅ and Pd₄₆Ag₅₄ can be obtained via adjusting the reaction parameters. For the formation process of the bimetallic PdAg nanoparticles, there have two-stage growth processes: firstly, nucleation a...