1. The following enzymes of the phosphorylated pathway of serine biosynthesis have been found in methanoland succinate-grown Pseudomona8 AM 1: phosphoglycerate dehydrogenase, phosphoserine-a-oxoglutarate aminotransferase and phosphoserine phosphohydrolase. Their specific activities were similar in the organism grown on either substrate. 2. A procedure for preparation of auxotrophic mutants of P...

Two variants of the methylotrophic bacterium W3A1, designated W3A1-S (slimy) and W3A1-NS (nonslimy), were compared with respect to their ability to grow in batch culture on the C1 substrates methylamine, methanol, and trimethylamine. Substrate utilization, cell density, pH, cellular and soluble polysaccharide production, and concentrations of the enzymes methylamine dehydrogenase, trimethylamin...

Poisonings from toxic alcohols, such as methanol (MeOH) are a serious toxicological concern worldwide. In an endemic methanol poisoning in 2012 in the Czech Republic 121 subjects had consumed adulterated beverages and 41 died from the poisoning. An additional 20 subjects still suffer from visual/CNS sequelae [1]. In addition to mass poisonings single cases of methanol poisoning had been publish...

Nicotine adenine dinucleotide-linked primary alcohol dehydrogenase and a newly discovered secondary alcohol dehydrogenase coexist in most strains of methanol-grown yeasts. Alcohol dehydrogenases from methanol-grown yeasts oxidize (--)-2-butanol preferentially over its (+) enantiomorph. This is substantially different from alcohol dehydrogenases from bakers' yeast and horse liver.

Formaldehyde is an intermediate formed during the metabolism of methanol or other methylated compounds. Many Gram-negative bacteria generate formaldehyde from methanol via a periplasmic pyrroloquinoline quinone (PQQ)-dependent dehydrogenase in which the alpha subunit of an alpha(2)beta(2) tetramer has catalytic activity. The genome of the facultative formaldehyde-oxidizing bacterium Rhodobacter...

Several studies have been made of the oxidation of C, compounds by micro-organisms grown on such compounds as sole source of carbon. In some cases, rather different results have been obtained by different workers. Kaneda & Roxburgh (1959) obtained evidence for an NAD+-linked methanol dehydrogenase in methanol-grown Pseudomonas PRL-W 4, whereas neither Harrington & Kallio (1960) nor Anthony & Za...

The conversion of carbon dioxide into important industrial feedstock is a subject of growing interest in modern society. A possible way to achieve this goal is by carrying out the CO2/methanol cascade reaction, allowing the recycle of CO2 using either chemical catalysts or enzymes. Efficient and selective reactions can be performed by enzymes; however, due to their low stability, immobilization...

In Methylobacterium extorquens AMI, genes encoding methanol dehydrogenase polypeptides are transcriptionally regulated in response to Cl compounds, including methanol (M. E. Lidstrom and D. I. Stirling, Annu. Rev. Microbiol. 44:27-57, 1990). In order to study this regulation, a transcriptional fusion has been constructed between a j-galactosidase reporter gene and a 1.55-kb XoI-Sall fragment of...

The largest of the gene clusters coding for proteins involved in methanol oxidation is the cluster mxaFJGIR(S)ACKLDEHB. Disruption of most of these genes leads to lack of growth on methanol. The previous results showed that the mutant lacking MxaD grows on methanol although at a low rate. This is explained by the low rate of methanol oxidation by whole cells. The specific activity of methanol d...

CO 2 can be enzymatically reduced to methanol in a cascade reaction involving three enzymes: formate-, formaldehyde- and alcohol dehydrogenase (FateDH, FaldDH, ADH).