The positioning of macrochromosomes of Bothrops jararaca and Bothrops insularis (Viperidae) was studied in undistorted radial metaphases of uncultured cells (spermatogonia and oogonia) not subjected to spindle inhibitors. Colchicinized metaphases from uncultured (spleen and intestine) and cultured tissues (blood) were also analyzed. We report two antagonic non-random chromosome arrangements in ...

Ma bamboo (Dendrocalamus latiflorus Munro) is a major species cultivated in southern China with high economic, ecological, and social value. However, highly dense forests reasonable structures have unclear adverse effects on light transmittance forest productivity that are not adequately understood. Here, we investigated varied light-intensity treatments during different phases of shoot emergen...

objective: mitochondria is a critical organelle within the cell and has important role in the oocyte development, thus the aim of this study was the comparison of mitochondrial distribution within the mouse oocyte at germinal vesicle (gv), germinal vesicle breakdown (gvbd) and metaphase ii (mii) stages using laser confocal microscopy (lscm). materials and methods: after superovulation of adult...

Although securin/separase/cohesion pathway was reported to regulate chromosome segregation during meiotic metaphase-to-anaphase transition, little biochemical evidence was provided. We recently found that oocytes could not progress beyond meiotic metaphase when ubiquitin-proteasome pathway was inhibited, but the mechanisms remain unclear. In the present study, we investigated the quantity of se...

Mitosis entails mechanistic changes required for maintaining the genomic integrity in all dividing cells. The process is intricate and temporally spatially regulated by ordered series of activation de-activation protein kinases. mitotic kinases ensure stepwise progression entry into mitosis after G2 phase cell cycle, followed prophase, pro-metaphase, metaphase, anaphase, telophase, subsequently...

Unfixed metaphase and non-metaphase cells were tested for their template activity with RNA polymerase. A device was used which disrupts the cell membrane by centrifugation, and which also ensures that the cells do not continue with their mitotic cycle during the transcription process. The template activity of acid/methanol fixed cells was also tested. None of the unfixed metaphase cells transcr...