The production of β-lactamase enzymes is one of the most distributed resistance mechanisms towards β-lactam antibiotics. Metallo-β-lactamases constitute a worrisome group of these kinds of enzymes, since they present a broad spectrum profile, being able to hydrolyze not only penicillins, but also the latest generation of cephalosporins and carbapenems, which constitute at present the last resou...

INTRODUCTION Acquired production of metallo-β-lactamases is an important mechanism of resistance in Pseudomonas aeruginosa. The objective of this study was to investigate the production of metallo-β-lactamase and the genetic diversity among ceftazidime-resistant P. aeruginosa isolates from State of Sergipe, Brazil. METHODS Metallo-β-lactamase was investigated using the disk approximation test...

β-Lactamases inactivate β-lactam antibiotics by hydrolysis of their endocyclic β-lactam bond and are a major cause of antibiotic resistance in pathogenic bacteria. The zinc dependent metallo-β-lactamase enzymes are of particular concern since they are located on highly transmissible plasmids and have a broad spectrum of activity against almost all β-lactam antibiotics. We present here essential...

Background and Aims: Pseudomonas aeruginosa is an opportunistic pathogen. The appearance of the Metallo-β-Lactamases genes and their spread among bacterial pathogens is a matter of concern with regard to the future of antimicrobial therapy.Antimicrobial resistance is an innate feature of bacterial biofilms. Biofilm formation is higher in MDR strains. The present study was undertaken with the ai...

Objective. To determine whether the genes whose products are annotated as «MBL-fold metallohydrolase superfamily» related to proteins of metallo-β-lactamase group. Materials and Methods. Sequences 7 were analyzed using following resources: ClustalW, IQ-TREE, iTOL. Selection oligonucleotides for real-time PCR was performed Primer-BLAST resource. The level gene expression assessed PCR. MIC MBC me...

BACKGROUND & OBJECTIVES Acinetobacter baumannii can cause a wide range of infections, including bacteremia, pneumonia, urinary tract infection, peritonitis, etc. This organism is becoming resistant to a large group of antibiotics, especially β-lactam antibiotics. The reason for multi-drug resistance may be the production of extended- spectrum β-lactamses (ESBLs), carbapenemases/metallo β-lactam...

β-Lactamase-mediated resistance is a growing threat to the continued use of β-lactam antibiotics. The use of the β-lactam-based serine-β-lactamase (SBL) inhibitors clavulanic acid, sulbactam, and tazobactam and, more recently, the non-β-lactam inhibitor avibactam has extended the utility of β-lactams against bacterial infections demonstrating resistance via these enzymes. These molecules are, h...

Pseudomonas aeruginosa is an opportunistic pathogen that causes frequently nosocomial infections, currently becoming more difficult to treat due to the various resistance mechanisms and different virulence factors. The purpose of this study was to determine the risk factors independently associated with the development of bacteremia by carbapenem-resistant P. aeruginosa, the frequency of virule...

Metallo β-lactamases is the name given to certain enzymes found in gram negative bacteria belonging to Enter-obacteriaceae which confers resistance to all β-lactam antibiotics including carbapenems except monobactams. They belong to Ambler molecular class B and Bush-Jacoby-Medeiros Group 3.1,2 Resistance to carbapenems due to production of metallo β-lactamases in Gram Negative organisms is an i...

AeromonAs are Gram-negative, facultative-anaerobic, non-sporeforming, glucose-fermenting, oxidaseand catalase-positive rods (Martin-Carnahan and Joseph 2005). Apart from fish, which are widely reported hosts for Aeromonads, insects, crustaceans, reptiles, birds and mammals were also found to harbour Aeromonas species, both in healthy and disease state (Pearson and others 2000, Turutoglu and oth...