نتایج جستجو برای: mes1

تعداد نتایج: 21  

Journal: :Genetics 2003
Zheng Xu Britton Lance Claudia Vargas Budak Arpinar Suchendra Bhandarkar Eileen Kraemer Krys J Kochut John A Miller Jeff R Wagner Michael J Weise John K Wunderlich James Stringer George Smulian Melanie T Cushion Jonathan Arnold

A bioinformatics tool called ODS3 has been created for mapping by sequencing. The tool allows the creation of integrated genomic maps from genetic, physical mapping, and sequencing data and permits an integrated genome map to be stored, retrieved, viewed, and queried in a stand-alone capacity, in a client/server relationship with the Fungal Genome Database (FGDB), and as a web-browsing tool for...

2013
Guijun Guan Yan Yan Tiansheng Chen Meisheng Yi Hong Ni Kiyoshi Naruse Yoshitaka Nagahama Yunhan Hong

Gene targeting (GT) by homologous recombination offers the best precision for genome editing in mice. nanos3 is a highly conserved gene and encodes a zinc-finger RNA binding protein essential for germ stem cell maintenance in Drosophila, zebrafish and mouse. Here we report nanos3 GT in embryonic stem (ES) cells of the fish medaka as a lower vertebrate model organism. A vector was designed for G...

Journal: :International journal of oncology 2009
Jinfeng Chen Lin Ye Lijian Zhang Wen G Jiang

Pigment epithelium-derived factor (PEDF) is an endogenous protein factor that has been shown to act as anti-angiognesis factor. The present study aimed to determine the direct biological effects of PEDF on lung cancer cells and deduce a clinical relevance in patients with lung cancer, major cause of death worldwide in which the knowledge of PEDF remains poor. We constructed a mammalian expressi...

2013
Yan Yan Ni Hong Tiansheng Chen Mingyou Li Tiansu Wang Guijun Guan Yongkang Qiao Songlin Chen Manfred Schartl Chang-Ming Li Yunhan Hong

BACKGROUND Gene targeting (GT) provides a powerful tool for the generation of precise genetic alterations in embryonic stem (ES) cells to elucidate gene function and create animal models for human diseases. This technology has, however, been limited to mouse and rat. We have previously established ES cell lines and procedures for gene transfer and selection for homologous recombination (HR) eve...

Journal: :Anticancer research 2010
Jinfeng Chen Lin Ye Fei Xie Yue Yang Lijian Zhang Wen G Jiang

BACKGROUND Lung cancer is the leading cause of cancer-related death in the world, with metastasis being the main reason for the mortality. As a member of the bone morphogenetic protein (BMP) family, BMP7 has important biological functions in malignant tumours. As yet, however, there is little knowledge of the role of BMP7 and its cellular function in lung cancer. This study aimed to investigate...

2014
Barbara Nuvoli Raffaela Santoro Simona Catalani Serafina Battistelli Serena Benedetti Franco Canestrari Rossella Galati

BACKGROUND CELLFOOD™ (CF) is a nutraceutical non-addictive, non-invasive, and completely non-toxic unique proprietary colloidal-ionic formula. Little is known about its effect on cancer cells in solid tumors. The aim of this study was to evaluate the effect that CF has on different cancer cell lines and the mechanism by which the nutraceutical works. METHODS The effect of CF on HFF (normal fi...

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