Mouse spleen cells which have been depleted of adherent cells do not respond to allogeneic lymphocytes in vitro. Their cytotoxic response can be restored by inclusion of mercaptoethanol in the medium. Mercaptoethanol is shown to have a stimulatory effect also on the response of normal (unseparated) spleen cells to alloantigens. The enhancement of the DNA-synthetic and cytotoxic response is simi...

the open pulled straw has been used effectively to vitrify preimplantation embryos because of geometricfeatures that allow rapid rates of temperature exchange. one possible inexpensive alternative to the openpulled straw are commercially-available microcapillary pipette tips commonly used for electrophoresis. themain purpose of this study was to compare the survival rates of in vitro produced b...

The aim of this study was to evaluate the effects of the addition of antioxidants (vitamin E, β- mercaptoethanol and their combination) to semen extender.  Progressive motility, viability and pH of fresh ram semen were examined. Ejaculate samples were collected with artificial vagina from three Ghezel-Merinose rams (3 years old) during non- breeding reproduction season. Semen was diluted with a...

BACKGROUND: Brucellosis is a febrile zoonotic infectionand has worldwide distribution among humans as well asanimals. Although the seroprevalence of brucellosis in variousanimals has been described in Iran, there is only one report onequine brucellosis in the region. OBJECTIVES: This study wascarried out to determine the seroprevalence of brucellosis inracing clubs and private horse owners in t...

The multiplicity reactivation of T4D phage irradiated with UV was inhibited by treatment of infected cells with 0.5 mM hydroquinone (HQ). HQ also inhibited genetic recombination of T4B rII mutants. The recombination frequency was reduced by treatment of infected cells with HQ in a buffer when compared with an untreated control. In addition to HQ, the effects of quinone, an oxide form of HQ, mer...

Human serum proteins were evaluated by sodium dodecyl sulfate polyacrylamide gel electrophoresis after protein denaturation in the presence or absence of 2-mercaptoethanol. Both electrophoretic techniques give characteristic and reproducible banding patterns and achieve a high degree of resolution within the limits of a one-dimensional separation. The major protein bands have been identified, a...