نتایج جستجو برای: melting curve analysis

تعداد نتایج: 2932383  

2010
Colin C Pritchard Laura Akagi Poluru L Reddy Loren Joseph Jonathan F Tait

BACKGROUND KRAS mutational analysis is the standard of care prior to initiation of treatments targeting the epidermal growth factor receptor (EGFR) in patients with metastatic colorectal cancer. Sensitive methods are required to reliably detect KRAS mutations in tumor samples due to admixture with non-mutated cells. Many laboratories have implemented sensitive tests for KRAS mutations, but the ...

Journal: :jundishapur journal of health sciences 0
farhad forouharmajd department of occupational health engineering, school of public health, isfahan university of medical sciences, isfahan, ir iran mitra shabab department of occupational health engineering, school of public health, isfahan university of medical sciences, isfahan, ir iran; department of occupational health engineering, school of health, ahvaz jundishapur university of medical sciences, ahvaz, ir iran; occupational health engineering department, school of health, ahvaz jundishapur university of medical sciences, ahvaz, ir iran. tel: +98-6133738269, fax: +98-33738282

conclusions according to the factory noise map, except for the control rooms with 81 dba noise, other cases of measuring stations were higher than the standard exposure of 85 dba noise. as a result, the implementation of control strategies, such as inspection and performance of control systems and insulation of the control room to prevent unnecessary exposure noise, are necessary. background st...

Journal: :Analytical biochemistry 2005
Paul T Monis Steven Giglio Christopher P Saint

Following the initial report of the use of SYBR Green I for real-time polymerase chain reaction (PCR) in 1997, little attention has been given to the development of alternative intercalating dyes for this application. This is surprising considering the reported limitations of SYBR Green I, which include limited dye stability, dye-dependent PCR inhibition, and selective detection of amplicons du...

Journal: :The Journal of Molecular Diagnostics 2010

2016
Antonios ZAmbounis Anastasios sAmArAs Aliki XAnthoPoulou maslin osAthAnunkul leonardo schenA Athanasios tsAftAris Panagiotis mAdesis

Zambounis A., Samaras A., Xanthopoulou A., Osathanunkul M., Schena L., Tsaftaris A., Madesis P. (2016): Identification of Phytophthora species by a high resolution melting analysis: an innovative tool for rapid differentiation. Plant Protect. Sci., 52. A new molecular method via the high resolution melting (HRM) analysis of the Ypt1 gene non-coding regions was validated for ten Phytophthora spe...

2013
Yanfang Zhu Qitian Wang Jin Hu Liwei Zhu Jiancheng Wang Shuijin Zhu Yajing Guan

Rice (Oryza sativa L.) is one of the most important crops in the world. It is essential to develop an efficient method to build the fingerprinting database of commercial hybrid rice cultivars and their parental lines in seed market for rapid and unambiguous cultivar identification and seed purity analysis as new similar cultivars increasing yearly. The objective of this investigation were to fi...

Journal: :Journal of clinical microbiology 2000
J Pietilä Q He J Oksi M K Viljanen

To differentiate the Borrelia burgdorferi sensu lato genospecies, LightCycler real-time PCR was used for the fluorescence (SYBR Green I) melting curve analysis of borrelial recA gene PCR products. The specific melting temperature analyzed is a function of the GC/AT ratio, length, and nucleotide sequence of the amplified product. A total of 32 DNA samples were tested. Of them three were isolated...

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