In the present study, we introduce a novel hybrid sandwich-ALISA employing chicken IgY and ssDNA aptamers for the detection of staphylococcal enterotoxin B (SEB). Cloning, expression and purification of the full length recombinant SEB was carried out. Anti-SEB IgY antibodies generated by immunizing white leg-horn chickens with purified recombinant SEB protein and were purified from the immunize...

African elephants of reproductive age in zoos are experiencing high rates of ovarian cycle problems (>40%) and low reproductive success. Previously, our laboratory found that 1/3 of acyclic females exhibit hyperprolactinaemia, a likely cause of ovarian dysfunction. This follow-up study re-examined hyperprolactinaemia in African elephants and found the problem has increased significantly to 71% ...

The ROSAT source RXJ1039.7-0507, known from its optical spectrum to be a Cataclysmic Variable star, is shown from high speed photometry to have an orbital period of 1.574 h. The system has a nearly sinusoidal photometric modulation with a range of 1.1 mag which we interpret as the reflection effect caused by a very hot white dwarf primary. This suggests that a nova explosion occured on the prim...

the purpose of this study was to determine the effects of official soccer competition on saliva cortisol, testosterone and immunoglobulin a in male players. for this purpose, 14 fixed players (age: 28.24±4.3 years) were equally selected from two opponent teams in 2009 elimination cup. saliva samples at the rest and immediately after the first half time, before and after the second half time wer...

background: kisspeptin-gpr54 system stimulates the hypothalamus-pituitary-gonadal (hpg) axis; dysfunction of the gene encoding the gpr54 receptor causes hypogonadism and infertility. opioid peptides inhibit the reproductive axis. peptide 234 is a gpr54 receptor antagonist and blocks the stimulatory effects of kisspeptin on hpg axis. objectives: interactions of morphine, kisspeptin and peptide 2...

Under the clinical impression that patients with chronic obstructive pulmonary disease (COPD) may demonstrate signs compatible with hypogonadism, we investigated whether oral glucocorticoid therapy is associated with testosterone deficiency. Thirty six men with COPD of whom 16 were receiving oral glucocorticoid medication (mean+/-SEM dose 9.4+/-1.1 mg prednisolone) were investigated in a cross-...

PURPOSE This study investigated whether hormones and pain perception are associated with exam anxiety, and also whether exam anxiety is affected by seasonal differences in testosterone and cortisol levels. MATERIALS AND METHODS Forty-six healthy males were recruited from a medical college. Anxiety was induced by having participants perform the Objective Structured Clinical Examination. Pressu...

Testosterone and cortisol in male marathon runners (n = 11) were determined in saliva samples (n = 28) collected during the three rest days preceding a competitive marathon and in the samples collected at 08.00h on the race day. An Eysenck Personality Inventory was completed on the first rest day and psychological state was assessed on rest days and on the morning of the marathon by completion ...

A simple, rapid, and ultra sensitive dispersive solid phase extraction based on nano graphene oxide was developed for simultaneous measurement of trace amounts of metformin (MET) and linagliptin (LIN) in plasma samples by HPLC-UV-Vis. Affecting factors on the extraction of these drugs, including adsorbent weight, extraction time, organic solvent type, desorption situations, and composition of s...

To examine the adaptations of the endocrine system to heavy-resistance training in younger vs. older men, two groups of men (30 and 62 yr old) participated in a 10-wk periodized strength-power training program. Blood was obtained before, immediately after, and 5, 15, and 30 min after exercise at rest before and after training and at rest at -3, 0, 6, and 10 wk for analysis of total testosterone...