The elaboration of enamel matrix glycoprotein was investigated in secretory ameloblasts of incisor teeth in 30-40-g rats. To this end, the distribution of glycoprotein was examined histochemically by the use of phosphotungstic acid at low pH, while the formation of glycoprotein was traced radioautographically in animals sacrificed 2.5-30 min after galactose-(3)H injection. Histochemically, the ...

Bovine nasal cartilage was studied by electron microscopy before and after extraction with 4 M guanidinium chloride or 1.9 M CaCl(2). These solvents removed matrix granules, basophilia, and 85% of the proteoglycan complex, measured as hexuronate. Simultaneously, many collagen fibrils were disaggregated into component microfibrils (approximately 40 A thick). In contrast to the above solvents, ex...

We have localized several major extracellular matrix protein receptors in the specific granules of human polymorphonuclear (PMN) and monocytic leukocytes using double label immunoelectron microscopy (IEM) with ultrathin frozen sections and colloidal-gold conjugates. Rabbit antibodies to 67-kD human laminin receptor (LNR) were located on the inner surface of the specific granule membrane and wit...

Anaerobic granular sludge is composed of multispecies microbial aggregates embedded in a matrix of extracellular polymeric substances (EPS). Here we characterized the chemical fingerprint of the polysaccharide fraction of EPS in anaerobic granules obtained from full-scale reactors treating different types of wastewater. Nuclear magnetic resonance (NMR) signals of the polysaccharide region from ...

Giant granules ( 1 sm in diameter), Auer rods, and unique giant granules that contained crystalloid material were investigated in monoblasts from a patient with acute monocytic leukemia using ultrastructural and cytochemical techniques. The latter granule type differed from abnormal granules previously described in leukocytes. In Wright’s stained preparations, these granules appeared to be crys...

—In this paper we propose a new method to represent information granules by Gaussian functional forms. First, the fuzzy granules are extracted from data by a fuzzy clustering algorithm. Then, they are properly represented by Gaussian functions determined by solving a constrained quadratic programming problem on membership values returned by the clustering algorithm. Simulation results show that...

Eosinophil (and heterophil) leukocytes of glycogen-induced rabbit peritoneal exudates were fixed for 1(1/2) min in 2% glutaraldehyde and examined for acid phosphatase activity both biochemically and cytochemically. Biochemical assays showed that enzymatic activity had been inhibited by only approximately 10% under these conditions. The cytochemical reaction in the eosinophil was confined to the...

We have used ultrathin cryosectioning and immunogold cytochemistry to study the position of a-granules in the endocytic and biosynthetic pathways in megakaryocytes and platelets. Morphologically, we distinguished three types of granules; so-called multivesicular bodies type I (MVB I) with internal vesicles only, granules with internal vesicles and an electron dense matrix (MVB II), and the a-gr...

We have used ultrathin cryosectioning and immunogold cytochemistry to study the position of alpha-granules in the endocytic and biosynthetic pathways in megakaryocytes and platelets. Morphologically, we distinguished three types of granules; so-called multivesicular bodies type I (MVB I) with internal vesicles only, granules with internal vesicles and an electron dense matrix (MVB II), and the ...