Introduction and Aim: In this study, the interaction between Acanthamoeba culbertsoni Shigella sonnei was analyzed. addition, effect of monosaccharides on cytotoxicity A. analyzed through with macrophages. particular, activity protease Materials Methods: Association tests were performed for trophozoites Escherichia coli DH5 alpha, proteolytic enzyme all these tests, monosaccharides, e.g., manno...

one of the important parts of innate immunity is complement system that occurs in three different ways; the classic, the alternative and the lectin pathway. the four pattern recognition molecules that have been identified till now are mannose binding lectin (mbl), a component of lectin pathway, and three ficolins (ficolin1,-2 and -3) which compound to the carbohydrates of the cell surface. mbl ...

Cell Tracking In article number 2207626, Jeff W.M. Bulte, Chao Wang, and Ali Shakeri-Zadeh review the occasional adverse effects encountered with magnetic labeling of cells for in vivo cell tracking using resonance imaging (MRI) particle imaging. Mesenchymal stem (MSCs) are abundant mannose residues on their membrane. Using mannose-sensitive chemical exchange saturation transfer MRI, MSCs is no...

mannose-binding lectin (mbl) is a protein of innate immune system that is involved in opsonization and complement activation. mbl deficiency is associated with predisposition to infectious diseases; however subnormal levels are also seen in healthy subjects. the aim of this study was to investigate the prevalence and clinical manifestation of mbl deficiency in patients with increased susceptibi...

Ascorbic acid (AsA) or Vitamin C is an antioxidant molecule and plays important role in many biological processes plants. GDP-D-mannose pyrophosphorylase (GMP VTC1) catalyzes the synthesis of GDP-D-mannose, which a precursor for AsA production used cell wall polysaccharide glycoprotein synthesis. In rice, OsVTC1 gene consists three homologs, including OsVTC1-1, OsVTC1-3 OsVTC1-8. this study, we...

Residual streams from lignocellulosic processes contain sugar mixtures of glucose, xylose, and mannose. Here, the industrial workhorse Corynebacterium glutamicum was explored as a research platform for rational utilization multiple substrate. The endogenous manA gene overexpressed to enhance mannose utilization. overexpression xylA Xanthomonas campestris in combination with xylB enabled xylose ...

Type I carbohydrate-deficient glycoprotein syndrome (CDGS) patients fail to add entire N-linked oligosaccharide chains to some serum glycoproteins. Here we show that four CDGS fibroblast cell lines have two related glycosylation abnormalities. First, they incorporate 3-10-fold less [3H] mannose into proteins, and, second, the size of the lipid-linked oligosaccharide precursor (LLO) is much smal...

When a derivatized oligosaccharide isolated from ovalbumin and containing 6 mannose residues was incubated with yeast membranes and GDP-mannose, two sets of products were obtained, a high molecular weight one containing about 25 mannose residues and a low molecular weight one consisting of compounds with 7, 8, and 9 mannose residues, respectively. When the low molecular weight products were rei...

results in this study, 45 (13.4 %) urine samples were positive for candida species (17 males and 28 females). the most common isolated yeast was candida albicans (34%), followed by c. glabrata (32.1%), c. tropicalis (9.4%), other candida species (22.6%), and rhodotorula species (1.9%). the mean serum levels of mbl were 0.85 ± 0.01 ng/ml and 1.02 ± 0.03 ng/ml among candiduric patients and contro...

When pig liver microsomal preparations were incubated with GDP-[(14)C]mannose, 10-40% of the (14)C was transferred to mannolipid and 1-3% to mannoprotein. The transfer to mannolipid was readily reversible and GDP was one of the products of the reaction. It was possible to reverse the reaction by adding excess of GDP and to show the incorporation of [(14)C]GDP into GDP-mannose. When excess of un...