The disialogangliosides GD2 and GD3 play a major role in the ability of human melanoma cells to attach to Arg-Gly-Asp-containing substrates such as fibronectin and vitronectin, since pretreatment of these cells with monoclonal antibodies to the oligosaccharide of GD2 and GD3 can inhibit their attachment and spreading on such adhesive proteins. This report demonstrates that human melanoma cells ...

Human melanoma cells (M21) actively attach and spread on a fibronectin substrate. Indirect immunofluorescence assays with specific monoclonal antibodies directed to the disialoganglioside GD2, the major ganglioside expressed on M21 melanoma cells, indicate that during the cell attachment process this molecule redistributes into microprocesses that make direct contact with the fibronectin substr...

Because certain antiganglioside monoclonal antibodies can facilitate antibody-dependent cellular cytotoxicity against GD2+ ganglioside-bearing human and canine tumor cells, we wished to determine if clinically relevant antiganglioside monoclonal antibodies (Mabs) could also fix canine complement to lyse tumor cells in vitro. Using flow cytometry, human tumor cell lines (M21 melanoma and OHS ost...

Let k be an algebraically closed field of characteristic 2. Consider F4 ⊂ k and P(F4) ⊂ P(k). Let P be the set of points and let P̌ be the set of lines in P(F4). Each set contains 21 elements, each point is contained in exactly 5 lines, and each line contains exactly 5 points. It is known that the group of automorphisms of the configuration (P, P̌) is isomorphic to M21 ·D12, where M21(∼ = PSL(3,F...

Using the published Kamiokande data of the multi-GeV atmospheric neutrinos, we have searched the optimum set of the neutrino oscillation parameters among three flavors. It is found that χ is minimized for (∆m21, ∆m 2 31) = (3.8×10−2 eV, 1.4×10−2 eV), (θ12, θ13, θ23) = (19,43,41) with χmin = 3.2 (42%CL). The sets of parameters (∆m21, ∆m 2 31) = (O(10−11eV2) or O(10−5eV2), O(10−2eV2)) which are s...

PURPOSE Metastatic melanomas are generally resistant to chemotherapy and radiation, even when wild-type for p53. These tumors often grow in small nests where many of the cells have little contact with extracellular matrix (ECM). Previous work showed that M21 melanomas undergo apoptosis in response to chemotherapy when cells are adherent to ECM but not in suspension. Thus, reduced integrin-depen...

We investigated the role of the integrins alpha v beta 3 and alpha v beta 5 in mediating vitronectin adhesion of three phenotypically distinct cell types. M21 human melanoma cells and H2981 lung carcinoma cells use both alpha v-containing integrins in adhering to vitronectin while UCLA-P3 lung carcinoma cells adhere exclusively with alpha v beta 5. Specifically, monoclonal antibodies directed t...

The simultaneous injection of monoclonal antibody 9.2.27, directed against a chondroitin sulfate proteoglycan preferentially expressed on human melanoma cells, and 2 X 10(7) mononuclear splenocytes, eradicated established, progressively growing human melanoma tumors in nude mice. Neither splenocytes nor antibody alone achieved significant tumor regression. The cells responsible for tumor elimin...

UNLABELLED Despite in vivo mapping of integrin αvβ3 expression being thoroughly investigated in recent years, its clinical value is still not well defined. For imaging of angiogenesis, the integrin subtype α5β1 appears to be a promising target, for which purpose we designed the PET radiopharmaceutical (68)Ga-aquibeprin. METHODS (68)Ga-aquibeprin was obtained by click-chemistry (CuAAC) trimeri...