Expression of eukaryotic proteins in E. coli often results in their aggregation. Proper folding and solubility of therapeutical proteins are the pre-requisite for their bioactivity. This is not achieved in cytoplasmic expression in E. coli because of the absence of disulfide bonds formation. A novel expression/secretion vector was constructed which exploited β-lactamase signal sequence to trans...

expression of eukaryotic proteins in e. coli often results in their aggregation. proper folding and solubility of therapeutical proteins are the pre-requisite for their bioactivity. this is not achieved in cytoplasmic expression in e. coli because of the absence of disulfide bonds formation. a novel expression/secretion vector was constructed which exploited β-lactamase signal sequence to trans...

We report on observations of six giants in the globular cluster M15 (NGC 7078) using the Subaru Telescope to measure neutron-capture elemental abundances. Our abundance analyses based on high-quality blue spectra confirm the star-to-star scatter in the abundances of heavy neutron-capture elements (e.g., Eu), and no significant s-process contribution to them, as was found in previous studies. We...

Vicriviroc (SCH 417690), a CCR5 receptor antagonist, is currently under investigation for the treatment of human immunodeficiency virus infection. The objective of this study was to identify human liver cytochrome P450 enzyme(s) responsible for the metabolism of vicriviroc. Human liver microsomes metabolized vicriviroc via N-oxidation (M2/M3), O-demethylation (M15), N,N-dealkylation (M16), N-de...

Despite decades-old predictions of the expected presence of dozens of cataclysmic variables in the cores of globular clusters, the number of irrefutable, out-bursting candidates is still barely a handful. Using multi-wavelength, multiepoch HST images we have produced outburst and quiescence light curves for the recently discovered large amplitude variable CV1 in the core of the post corecollaps...

Up-to-date isochrones, zero-age horizontal branch (ZAHB) loci, and evolutionary tracks for core Heburning stars are applied to the color-magnitude diagrams (CMDs) of M3, M15, and M92, focusing in particular on their RR Lyrae populations. Periods for the aband c-type variables are calculated using the latest theoretical calibrations of log Pab and log Pc as a function of luminosity, mass, effect...

A Gram-negative, short rod-shaped bacterium, strain GA2-M15(T), was isolated from a sea-sand sample at Homi Cape, Pohang city, Republic of Korea. 16S rRNA gene sequence analysis demonstrated that this isolate was unique, showing 95.9 % sequence similarity to the type strain of Thalassobacter stenotrophicus and similarities of 94.0-95.2 % to the type strains of species of the genera Octadecabact...

High resolution spectra of 110 selected red giant stars in the globular cluster M15 (NGC 7078) were obtained with Hectochelle at the MMT telescope in 2005 May, 2006 May, and 2006 October. Echelle orders containing Hα and Ca II H & K are used to identify emission and line asymmetries characterizing motions in the extended atmospheres. Emission in Hα is detected to a luminosity of log(L/L⊙) = 2.3...

BACKGROUND Antibiotic resistance is widespread among diarrheagenic Escherichia coli in developing countries, where the overuse of antibiotics is common. Information regarding β-lactamases, especially Extended-Spectrum β-Lactamases (ESBLs) in diarrheagenic pathogens should be considered in clinical management when an optimal treatment is needed. OBJECTIVES The main objective of this study was ...