Grapevine (Vitis vinifera cv. Touriga) callus cell walls contain a high level of the monomeric extensin, GvP1. Hydrogen peroxide stimulus of these cultures causes the rapid loss of monomeric GvP1, concomitant with marked increases in insoluble GvP1 amino acids and wall resistance to digestion by fungal lytic enzymes. JIM11 immunolocalization studies indicated that monomeric and network GvP1 wer...

90 Virulence Volume 4 Issue 1 Phage lytic enzymes have potential as new inroads toward novel antibiotics. Until now this approach has only been promising for Grampositive bacteria because in Gram-negatives the target of lytic action is protected by an outer envelope. Information gleaned from the structural studies of two plague proteins— pesticin and FyuA—allowed us to engineer a “hybrid” prote...

90 Virulence Volume 4 Issue 1 Phage lytic enzymes have potential as new inroads toward novel antibiotics. Until now this approach has only been promising for Grampositive bacteria because in Gram-negatives the target of lytic action is protected by an outer envelope. Information gleaned from the structural studies of two plague proteins— pesticin and FyuA—allowed us to engineer a “hybrid” prote...

Tetanolysin, partially purified, caused the lysis of human and rabbit platelets, as determined by a decrease in the optical density of platelet suspensions and the release of serotonin, enzymes, and protein. This lytic activity was neutralized by antitoxin. In addition, a suspension of the lysosome-containing large granule fraction of rabbit liver released hydrolytic enzymes when exposed to tet...