BACKGROUND Lysozyme is an antimicrobial innate immune molecule degrading peptidoglycan of the bacterial cell wall. Lysozyme shows the ubiquitous expression in wide varieties of species and tissues including the tubotympanum of mammals. We aim to investigate the effects of lysozyme depletion on pneumococcal clearance from the middle ear cavity. METHODS Immunohistochemistry was performed to loc...

Lysozyme, a low-molecular weight basic protein, is an important component of the antibacterial system in milk. Lysozyme activity is higher in buffalo milk (60±3.9r 10 units/ml) than in bovine milk (29.1±1.5r10 units/ ml). Buffalo colostrum contains five-times more lysozyme activity than mature milk (Priyadarshini & Kansal, 2002a). Lysozyme activity in buffalo milk is not influenced by the parit...

The mouse sensitized by subcutaneous (sc) injection of lysozyme in emulsion of Freund's complete adjuvant (FCA) was shown by a modified footpad test to develop three kinds of hypersensitivities. Injecting lysozyme in 2.5-mul emulsion of Freund's incomplete adjuvant (FIA) into the footpad elicited strong footpad swelling in 30 min (anaphylactic reaction), in 3 hr (Arthus-type reaction) and in 24...

in the present study, the interaction between nano-tio2 and lysozyme was investigated by the method of uv-vis detection and fluorescence spectroscopic techniques. the thermal denaturation of lysozyme has been investigated in the presence and absence of nano-tio2 over the temperature range (293-373) k in different buffer and ph, using temperature scanning spectroscopy. the presence of nano-tio2 ...

the present experiment was undertaken to study the effect of different dosages of β-glucan on the growth, survival and physiological responses in juvenile striped catfish ( pangasianodon hypophthalmus ). over nine weeks of feeding fish with a diet containing 0, 0.5, 1 and 2% β-glucan (as control, g1, g2 and g3 group, respectively), some blood parameters such as serum lysozyme activity, total pr...

Properties of Crystalline Lysozymes............................................................ 83 Non-enzymic Reactions of Lysozyme ........................................................... 84 Agglutination of Bacterial Cells............................................................. 84 Formation of Complexes with Acidic Substances.............................................. 84 Susceptib...

Intracellular lysozyme (muramidase) activity was measured in leukemic blasts and mature neutrophilic granulocytes from 20 patients with acute myeloblastic and myelomonocytic leukemia and in 11 patients with acute lymphoblastic leukemia after differential centrifugation of cells in Ficoll and extraction of lysozyme with n-butanol. Considerable abnormalities in cellular lysozyme activity were fou...

Lysozyme activity in buffalo milk in relation to the period of lactation, parity of animal, weather conditions and udder infections was studied. Effect of storage and heat processing of milk on lysozyme activity was determined. Lysozyme activity was higher in buffalo milk than in cow milk. Buffalo colostrum showed lysozyme activity 5 times of that in mature milk. Lysozyme activity in buffalo mi...

The lysozyme content of human cartilage was measured by incubation of lyophilized, powdered cartilage in a variety of buffers and salt solutions, and the factors controlling the binding of lysozyme within cartilage were studied. Lysozyme was extracted from hyaline cartilage by buffers of pH greater than 9.0 by solutions 1 M in monovalent cations, and by solutions 0.12-0.40 M in divalent cations...

Several previous findings have suggested that the cationic nature of lysozyme is a major factor in its bactericidal activity. Since a number of cationic proteins or peptides have been reported to cause membrane damage in bacteria, we investigated the effect of lysozyme on glucose fermentation and intracellular pH and K+ in Streptococcus mutans under conditions in which lysis does not occur. Res...