introduction: the aim of this study was to investigate the effect of the hsp-70 (heat shock protein-70) induction in the lysate of heat shocked tumor cells on splenocyte proliferation, nitric oxide production by peritoneal macrophages, and fibrosarcoma tumor size reduction in balb/c mice. material and methods: wehi 164 cells (mouse fibrosarcoma cell line) were injected subcutaneously into the r...

Platelet aggregation activity due to platelet-activating factor (PAF) was detected at high molecular weight (HMW) and low molecular weight fractions after gel-filtration chromatography of cell lysate of endothelial cells. [3H]PAF added to the cell lysate was similarly distributed after chromatography. The radioactivity associated with HMW fraction was not reduced by digesting the lysate with tr...

In this research, we investigated the cytotoxic mechanisms of Cochlodinium polykrikoidescell lysate on isolated rat liver hepatocytes.This micro algae is responsible for a severe and widespread harmful algal bloom in the Persian Gulf and Gulf of Oman (2008-2009). Isolated hepatocytes were obtained by collagenase perfusion of Sprague-Dawley rat liver.According to our results, incubation of algal...

protein hydro-lysate was prepared from gold striped sardine (sardinella gibossa) by papain. hydrolysis conditions (time, temperature, and enzyme activity) were optimized by response surface methodology (rsm) using a factorial design and anti-oxidative activity was the dependent variable. results of the total determination coefficient, adjusted r2 and predicted r2 indicating the validity of pred...

Background: Malignant mesothelioma (MM) is a highly aggressive, incurable asbestos-induced cancer for which treatment options are limited. Surgical resection can reduce tumour burden, but patients ultimately succumb to disease due to reoccurrence of unresectable tumour, highlighting the need for new treatment modalities. In this study we describe the use of an easily translatable heat shock (HS...

Although tumor lysate contains all the potential helper and killer epitopes capable of stimulating T cells, it is difficult to use as a cancer vaccine because it suppresses dendritic cell (DC) function. We report that wild-type baculovirus possesses an adjuvant effect to improve the immunogenicity of tumor lysate. When mice were administered CT26 tumor cell lysate combined with baculovirus intr...

Dendritic cells (DCs) are potent antigen-presenting cells that are capable of priming systemic antitumor immune response. Here, we evaluated the combined effectiveness of tumor lysate-pulsed DC immunization and interleukin (IL)-12 administration on the induction of antitumor immunity in a mouse hepatocellular carcinoma (HCC) model. Mouse DCs were pulsed with lysate of BNL 1ME A.7R.1 (BNL), a BA...

objective: dendritic cells (dcs) are essential for the activation and polarization of t cells during an adaptive immune response. in this research we investigated the effect of the lymphoide dcs pulsed with heat-treated tumor lysate (htl) as a vaccine in tumor immunotherapy. materials and methods: the balb/c mice were injected subcutaneously in the right flank with wehi-164 fibrosarcoma cells ...

Background and Aim: Pseudomonas aeruginosa is an important ubiquitous and especially common pathogen in the hospital. Exotoxin A that encoded by exoA gene has a role in pathogenesis of this bacterium. Today, probiotics are widely used in the treatment and prevention of diseases. The present study aimed to study the Saccharomyces cerevisiae S3 effect on the expression of exoA gene. Materials an...

Appropriate treatment of non-healing diabetic foot ulcers is challenging as the whole skin repair process complicated. Current therapeutic strategies have to be evolved for better efficacy. Staphylococcus epidermidis (S. epidermidis) a commensal having probiotic properties. The wound healing potential its lysate was explored using biopsies. Full-thickness skin, obtained from individuals with an...