Wounding lettuce (Lactuca sativa L., var. Longifolia) leaves induced an eight-fold increase in the activity of phenylalanine ammonia-lyase (PAL; EC 4.3.1.5), and the subsequent accumulation of phenolic compounds and tissue browning at 10 C. PAL is a key enzyme in the synthesis of phenolic compounds. A PAL cDNA was previously isolated by reverse-transcription PCR using total RNA from wounded let...

Novel hybrid magnetic cross-linked enzyme aggregates of phenylalanine ammonia lyase (HM-PAL-CLEAs) were developed by co-aggregation of enzyme aggregates with magnetite nanoparticles and subsequent crosslinking with glutaraldehyde. The HM-PAL-CLEAs can be easily separated from the reaction mixture by using an external magnetic field. Analysis by scanning electron microscopy (SEM) and confocal la...

The herbicide glyphosate, N-(phosphonomethyl)glycine, induced phenylalanine ammonia-lyase (PAL) activity in the roots of dark-grown maize seedlings. This enzyme induction was shown to precede glyphosate-reduced fresh weight gain in the roots by 24--48 h. Feeding aromatic amino acids with glyphosate further inhibited growth while slightly lowering glyphosate-enhanced PAL activity. Soluble protei...

A separable and highly-stable enzyme system was developed by adsorption of phenylalanine ammonia lyase (PAL) from Rhodotorula glutinis in amino-functionalized macroporous silica gel and subsequent enzyme crosslinking. This resulted in the formation of cross-linked enzyme aggregates (PAL-CLEAs) into macroporous silica gel (MSG-CLEAs). The effect of adsorptive conditions, type of aggregating agen...

Due to high water content and perishability, the raspberry fruit is sensitive postharvest fungal contamination losses. In this study, chitosan was used as an edible coating increase storage of raspberries, nanotechnology efficiency. The treated with emulsion containing nanoparticles (ECNPC) at 0, 2.5, 5 g L?1, stored for 9 d. Decay extension rate, phytochemical contents, including total phenoli...

Phenylketonuria (PKU) is a metabolic disorder, in which loss of phenylalanine hydroxylase activity results in neurotoxic levels of phenylalanine. We used the Pah(enu2/enu2) PKU mouse model in short- and long-term studies of enzyme substitution therapy with PEGylated phenylalanine ammonia lyase (PEG-PAL conjugates) from 4 different species. The most therapeutically effective PAL (Av, Anabaena va...

Pharmacological evidence implicates trans-cinnamic acid as a feedback modulator of the expression and enzymatic activity of the first enzyme in the phenylpropanoid pathway, L-phenylalanine ammonia-lyase (PAL). To test this hypothesis independently of methods that utilize potentially non-specific inhibitors, we generated transgenic tobacco lines with altered activity levels of the second enzyme ...

Phenylketonuria (PKU) is a genetic metabolic disease in which the decrease or loss of phenylalanine hydroxylase (PAH) activity results in elevated, neurotoxic levels of phenylalanine (Phe). Due to many obstacles, PAH enzyme replacement therapy is not currently an option. Treatment of PKU with an alternative enzyme, phenylalanine ammonia lyase (PAL), was first proposed in the 1970s. However, iss...

BACKGROUND α-amidation is a final, essential step in the biosynthesis of about half of all peptide hormones and neurotransmitters. Peptidylglycine α-amidating monooxygenase (PAM), with enzymatic domains that utilize Cu and Zn, is the only enzyme that catalyzes this reaction. PAM activity is detected in serum, but its significance and utility as a clinical biomarker remain unexplored. METHODS ...

Abstract The cereal aphids Rhopalosiphum padi L. are serious pests on grain crops, reducing the quality and yield by direct feeding damage virus transmission. changes in physiological biochemical parameters of two wheat cultivars under aphid stress were investigated to understand aphid-resistance mechanisms. activity levels phenylalanine ammonia-lyase (PAL), peroxidase (POD), tyrosine (TAL), Ca...