Superoxide Detection To the Editor: The recent review by Tarpey and Fridovich published in Circulation Research1 mostly contains interesting and sound material; however, I would like to comment on the section entitled, “Superoxide Detection, Chemiluminescence Reactions.” Basing mainly on their own data,2 authors maintain that lucigenin-amplified chemiluminescence (LucCL) overestimates the rate ...

Lucigenin and paraquat are similar in that each can be taken into Escherichia coli and can then mediate O2.- production by cycles of univalent reduction, to the corresponding monocation radical, followed by autoxidation. Thus, both compounds caused induction of enzymes that are regulated by the soxRS regulon. The lucigenin cation radical has the added property of reacting with O2.-, in a radica...

The stimulation of neutrophils by formylmethionyl-leucyl-phenylalanine results in a bimodal luminol-dependent chemiluminescence pattern. We observed, however, only a single peak chemiluminescence pattern in the response to the peptide when we used lucigenin as an amplifying substance. We suggest that lucigenin, the larger molecule, (510 daltons; luminol is 177 daltons) only exerts an extracellu...

BACKGROUND The anti-inflammatory effects of a homeopathic remedy, Traumeel S, have been observed in experimental and clinical studies; however, its antioxidant properties have not been elucidated. The aim of the present study was to evaluate the antioxidant effects of Traumeel S on peripheral blood neutrophils in patients with periodontitis. MATERIAL/METHODS The study was performed using veno...

In this study we have examined the biochemical attributes of the redox systems that regulate human sperm function using 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulphophenyl)-2H-tetrazolium, monosodium salt (WST-1), lucigenin and luminol-peroxidase as probes. WST-1 was readily reduced by human spermatozoa in the presence of an intermediate electron acceptor (IEA) or NAD(P)H. The IEA-mediated...

The method using lucigenin-dependent phagocytic chemiluminescence is described for the assessment of alveolar macrophage metabolic activity in response to stimulation by opsonised particles or soluble agents. The requirement for superoxide anion (O2-) in the production of chemiluminescence is suggested by inhibition (95%) using superoxide dismutase. The results obtained are correlated with thos...

BACKGROUND Different types of reactive oxygen metabolites (ROMs) are known to be involved in carcinogenesis. Several studies have emphasized the formation of ROMs in ischemic tissues and in cases of inflammation. The increased amounts of ROMs in tumor tissues can either be because of their causative effects or because they are produced by the tumor itself. Our study aimed to investigate and com...

the reaction of hydrogen peroxide and  pheny -9-acridinecarboxylate in the presence of rhodamin b as a fluorophore leads to the emission of either bright blue light  or bright red light, depending on the structure of fluorescer and reaction conditions. the light emitter responsible for the chemi luminescence (cl) reaction is acridone and that the blue light produced with out the fluorescers is ...