A microfluidic bioreactor for cell co-culture has been developed to study the cytotoxicity of xenobiotics in multiorgan interaction models. In this paper, we investigate the drug metabolism of ifosfamide, an anticancer drug, in liver and its impact on kidney. HepG2/C3A (liver) and MDCK (kidney) cells are cultured in two separate PDMS chambers connected by a perfusion loop mimicking blood flow. ...

Abstract In isolated environments such as stand-alone bioreactor systems, microbial communities change their composition stochastically. Starting from identical inocula and with reactor operation, will always evolve into different compositions. Here, we have developed a loop-designed system that synchronizes stabilizes multiple in connected reactors over long periods of time.

In vitro arterial flow bioreactor systems have been used in tissue engineering as models to investigate the response of endothelial cells to shear. However, the degree to which such models can reproduce physiological flow has not been quantified. Furthermore, previous works have assumed a Poiseuille flow profile for the calculation of wall shear stresses (WSS) experienced by the endothelial lay...

Identification based PID tuning is studied. The proposed approach consists of the identification of linear or nonlinear process model and model based control design. The identification test can be performed in both open loop and closed-loop. The so-called ASYM method is used to solve the identification problem. The method identifies a low order process model with a quantification of model error...

We consider a control problem for a single bioreaction occuring in a continuous and well mixed bioreactor, assuming that the bioreaction’s kinetics is not represented by a validated model. We develop a nonlinear controller and prove the global asymptotic stability of the closed loop system towards the equilibrium corresponding to the set point. Since this control law needs the knowledge of some...

Quality, quantity, and economy of the fermentation product depend on transient response steady-state process. Due to its simple construction, an industrial batch bioreactor cannot be equipped with a closed-loop control system that applies adding or removing substances during fermentation. This theoretical practical study displays how can controlled, in this case by altering rotational speed sti...