Hypothesis / aims of study Nitric oxide (NO) has been recognized as an important neurotransmitter in the lower urinary tract. Previous studies have shown the presence of NO synthases (NOS) in urothelium and detrusor smooth muscle (DSM) in different animal species. Recently, long-term NO blockade has been shown to cause bladder dysfunction and detrusor overactivity in rats [1,2]. However, the me...

During the experiment, modeling of endothelial dysfunction male rats was performed by intraperitoneal administration L-NAME at a dose 25 mg/kg for 7 days, and same female bilateral ovarioectomy further 7. The deficiency nitric oxide as result NO-synthase blockade accompanied impairment endotheliumdependent independent vasodilatation estimated in pharmacological tests, which expressed increasing...

The role of fatty acyl CoA synthetase (FACS) in ischemia/reperfusion (I/R) injury has not been well established. Our earlier studies showed that triacsin C, a selective FACS inhibitor, decreases endothelial nitric oxide synthase (eNOS) palmitoylation and increases nitric oxide (NO) in cultured human coronary endothelial cells. In the present study, we tested the hypothesis that triacsin C would...

Cardiometabolic alterations are frequent in patients with heart failure preserved ejection fraction (HFpEF). We recently demonstrated sinoatrial node (SAN) dysfunction limits the chronotropic response two distinct animal models of HFpEF; however, our new findings suggest a relationship between metabolism and SAN dysfunction. To identify metabolically-driven changes that facilitate HFpEF. Male C...

The etiology of neuronal death in neurodegenerative diseases, including Huntington′s disease (HD), is still unknown. There could be a complex interplay between altered energy metabolism, excitotoxicity and oxidative stress. Our aim was to examine the effects of intrastriatal injection of a selective inhibitor of neuronal nitric oxide synthase, 7 nitro indazole (7-NI), and non-specific potent ni...

347 Abstract: In this study the effect of diphtheria toxin (DT) on nitric oxide (NO) production from RAW 264.7 murine macrophages was investigated. Griess reagent was used to determine NO production by measuring nitrite levels in the culture supernatants. Corynebacterium diphtheriae G12/6 strain-produced DT (Limes flocculation activity and immunodiffusion assays were positive) demonstrated a do...

Mizuta, Yohei, Toku Takahashi, and Chung Owyang. Nitrergic regulation of colonic transit in rats. Am. J. Physiol. 277 (Gastrointest. Liver Physiol. 40): G275–G279, 1999.— Nitric oxide has been shown to be an inhibitory neurotransmitter in the mammalian colon, although its role in colonic transit remains unclear. We investigated the effect of the nitric oxide biosynthesis inhibitor NG-nitro-L-ar...