Transcripts of Leptospira 16S rRNA, FlaB, LigB, LipL21, LipL32, LipL36, LipL41, and OmpL37 were quantified in the blood of susceptible (hamsters) and resistant (mice) animal models of leptospirosis. We first validated adequate reference genes and then evaluated expression patterns in vivo compared to in vitro cultures. LipL32 expression was downregulated in vivo and differentially regulated in ...

Leptospirosis, a disease with protean manifestations, is caused by Leptospira species complex. A definite diagnosis of leptospirosis during the first week of the illness is a major challenge to investigating clinical laboratories. PCR is a rapid, sensitive and specific means of diagnosing Leptospira infections in the early phase of the disease. In this study, the applicability of routine PCR as...

Leptospirosis is an emerging infectious disease and is considered to be the most widespread zoonotic disease in the world. It can be misdiagnosed because manifestations of this febrile disease vary from mild flu-like symptoms to severe illness involving vital organs such as the liver and lungs. Therefore, accurate diagnosis for differentiation of leptospirosis from other pyrogenic infections pr...