نتایج جستجو برای: lipase activity
تعداد نتایج: 1145290 فیلتر نتایج به سال:
Background: Despite several studies many questions remain regarding how varicocele develops its adverse effect on testicular tissue. Spermatogenesis cell lineage needs carbohydrates as a main source of energy for cell division. Any disruption in glucose transporting system in seminiferous tubules results in remarkable decrease in germinal cells biological function. Therefore present study was c...
Pseudomonas aeruginosa EF2, ATCC 9027 and ATCC 19660 were grown in a continuous culture under Tween 80 (polyoxyethylene sorbitan monooleate) limitation and optimum conditions (pH 6.5, 37°C at dilution rate of 0.05/h). Culture supernatants were carefully removed and stored at -20°C. To purify the lipases, the culture supernatant was reduced in volume to approximately 10 mL by an ultrafiltra...
an aerobic, meophilic and lipolitic bacillus.sp bacterium (lipase activity 40 u/ml) was isolated from the olive extract. this isolate was identified based on morphological and biochemical characterization and 16s rrna gene sequence. effect of the solid substrate (pomegranate seed ground, grape seed ground and coriander seed ground as a solid substrate), the substrate particle size, moisture con...
using enrichment procedures, 45 organic solvent-tolerant lipase producer bacterial strains were screened from areas contaminated by oil and organic solvents. among the strains, 15 isolates exhibited extreme stability toward organic solvents and high lipolytic activity. the neb-1 isolate which was later identified as pseudomonas sp. strain neb-1 by biochemical tests and 16s rdna gene sequ...
background and objectives: lipases are valuable biocatalysts which are widely used in the detergent, food, dairy and pharmaceutical industries. the aims of the present study included the isolation of a lipase-producer from industrial zones and the partial characterization of the enzyme. materials and methods: a number of bacteria were isolated from sites related to the oil industries. an isolat...
Pseudomonas aeruginosa BBRC-10036 was used for lipase production. The organism secreted the enzyme extracellulary. In order to purify the enzyme, precipitation was done first, and then this lipase has been purified by Ion exchange Chromatography leading to 2.3-fold purification and 11.47% recovery. Lipase from P.aeruginosa was entrapped into Ca-alginate gel beads and effect of independent varia...
Enzymes of wheat flour have an important role in production of various cereal products, especially bread, and therefore accurate measurement of enzymatic activity along with improvement of this characteristic is the basis of most studies in baking industry. In the present work, physical properties of three wheat varieties (Mahdavi, Kavir and M7318 that are cultivated in Isfahan province) and ch...
Enzymes of wheat flour have an important role in production of various cereal products, especially bread, and therefore accurate measurement of enzymatic activity along with improvement of this characteristic is the basis of most studies in baking industry. In the present work, physical properties of three wheat varieties (Mahdavi, Kavir and M7318 that are cultivated in Isfahan province) and ch...
Cloning and sequencing of a lipase gene from an indigenous Bacillus pumilus, strain F3, revealed an open-reading frame of 648 nucleotides predicted to encode a protein of 215 residues. Sequence analysis showed that F3 lipase contained a signal peptide composed of 34 amino acids with an H domain of 18 residues. A tat-like motif was found in the signal peptide similar to some other Bacillus pumil...
The objective of the present study was to determine the antilipidperoxidation and in vitro α-amylase and lipase inhibitory activity of the Boswellia ovalifoliolata Bal Henry (BOB) extract. The polyphenolic compounds, flavonoids, and tannins content of the extracts were estimated by spectrophotometry. Antioxidant activity on goat liver lipid peroxidation and linoleic acid was d...
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