Background: Coronary artery disease (CAD) is one of the leading causes of death and disability around the world. Interaction between genetic and environmental factors determines susceptibility of an individual to develop coronary artery disease . Lipoprotein lipase (LPL) play an important role in the metabolism of HDL-C ( High Density Lipoprotein Cholesterol ), LDL-C (Low Density Lipoprotein Ch...

Efforts to express lipase in the periplasmic space of Escherichia coli have so far been unsuccessful andmost of the expressed recombinant lipases accumulate in the insoluble cell fraction. To evaluate the role ofnative and heterologous signal peptides in translocation of the lipase across the inner membrane of E. coli,the lipase gene (btl2) was cloned downstream of the native ...

background: coronary artery disease (cad) is one of the leading causes of death and disability around the world. interaction between genetic and environmental factors determines susceptibility of an individual to develop coronary artery disease . lipoprotein lipase (lpl) play an important role in the metabolism of hdl-c ( high density lipoprotein cholesterol ), ldl-c (low density lipoprotein ch...

Genetic hyperchylomicronemia is a rare autosomal recessive disorder of lipoprotein metabolism estimated to affect approximately one per million individuals. We report a case with a rare mutation identified. It’s a genetic chylomicronemia in a Moroccan newborn baby, with massive hypertriglyceridemia and clinical signs of acute pancreatitis. She was a newborn female, first-degree of consanguineou...

an aerobic, meophilic and lipolitic bacillus.sp bacterium (lipase activity 40 u/ml) was isolated from the olive extract. this isolate was identified based on morphological and biochemical characterization and 16s rrna gene sequence. effect of the solid substrate (pomegranate seed ground, grape seed ground and coriander seed ground as a solid substrate), the substrate particle size, moisture con...

Several lipases and their cofactors are involved in the absorption, transport, storage, and mobilization of lipids. As part of an effort to examine the role of these enzymes in plasma lipid metabolism and genetic susceptibility to atherosclerosis, we report the chromosomal mapping of their genes in mouse. Restriction fragment length variants for each gene were identified, typed in an interspeci...

conclusions results of this study could be used to find suitable targets for inverse metabolic engineering and site-directed mutagenesis to achieve stable and high-level extracellular lipase production in y. lipolytica and other yeasts. results two nucleotide substitutions were found in the extracellular lipase lip2 orfs of y. lipolytica u6 mutant strain compared to the wild-type strain. in thi...

Underwater volcano is an example of a natural habitat thermophilic bacteria, such as hydrothermal vent Kawio in North Sulawesi. The focus this research was to screen the bacterial isolates originally from for production thermostable lipase. In study, lipase gene selected isolate cloned, sequenced, and silico analysis performed predict characteristics protein. One three examined, showed positive...

The expression of lipase from Pseudomonas sp. strain KWI-56 (recently reclassified as Burkholderia cepacia) had been found to be dependent on an activator gene (act) downstream of its structural gene (lip). In this work, the mature lipase was synthesized in an enzymatically active form with a cell-free Escherichia coli S30 coupled transcription-translation system by expressing a recombinant lip...

Het onderzoek beschreven in dit proefschrift werd uitgevoerd op de afdeling Biochemie Cardiovascular Research School COEUR en de afdeling interne geneeskunde van de Erasmus Universiteit te Rotterdam. 1.0 Introduction 7 1.1 Role of Hepatic lipase in lipoprotein metabolism 8 1.1.1 Hepatic lipase 8 1.1.2 Hepatic lipase and reverse cholesterol transport 10 1.1.3 Hepatic lipase and steroidogenesis 1...