materials and methods a total of 228 fecal samples were collected from residents of rural areas of hamadan, west of iran. each fecal sample was divided into two parts, one kept frozen at -20˚c for ag-capture elisa and the other in 10% formalin for the ap staining method. cryptosporidium ag-detection elisa procedure was performed according to the manual of the manufacturer. the preserved samples...

background herpes simplex virus type 1 and type 2 (hsv-1 and hsv-2) are common infectious agents worldwide. data on prevalence of hsv-1 and hsv-2 infections are limited in asia, especially in iran. our study aimed on determination of seroepidemiology of hsv-1 and hsv-2 infections in asymptomatic healthy students of islamic azad university of kazeroun, fars province, southwest of iran. patients ...

background: aflatoxin m1 (afm1) as a carcinogenic and toxic compound has considerable significance from public health viewpoint. the aim of this study was to determine the level of afm1 in pasteurized and uht milk in west-azerbaijan province of iran. methods: a total of 360 commercial milk samples including 220 pasteurized milk and 140 ultra-high-temperature (uht) milk were purchased randomly f...

cholera toxin b subunit (ctb) has been extensively studied as an immunogen, adjuvant, and inducer of oral tolerance in many investigations. production of ctb has been carried out in the bacterial, plant, insect and yeast expression systems. in this study the expression of the ctb containing a 6xhis-tagged was performed by escherichia coli (e.coli) m15. the yield of purified pentameric recombina...

results the optimal conditions were considered to perform elisa. comparison between results obtained from optimized elisa kit and commercial elisa kit showed a good agreement. background haemophilus influenzae type b (hib) infection has high morbidity and mortality rate, especially in children under 5 years of age. enzyme-linked immunosorbent assay (elisa) technique is the most used method to d...

alfalfa mosaic virus (almv) is one of the most conspicuous alfalfa viruses in the world. in order to identify the distribution, host range and molecular characterization of alfalfa mosaic virus in kerman province, 514 alfalfa samples showing mosaic, calico, mottle and malformation symptoms as well as 66 potato samples showing calico symptoms were collected from alfalfa and potato fields in kerm...

background: pan-igg specific monoclonal antibodies (mabs) are essential tools for assessment of humoral immunity, immune deficiency and gammopathy. in this study, four hybridoma clones producing mabs with different specificities for human igg isotypes were established. methods: splenocytes from balb/c mice immunized with fc fractions of human igg were fused with sp2/0 myeloma cells. hybridoma c...

universal vaccination of neonates and children against diphtheria, tetanus and pertussis has had a tremendous impact on the control of these infectious diseases worldwide. immunization by the triple diphtheria, tetanus and whole cell pertussis vaccine (dtwp) has been applied in iran for almost 50 years. periodic assessment of immunogenicity of this vaccine is an important aspect of successful m...

cystic echinococcosis is an important zoonosis in the sheep rising areas of iran. to develop a simple and reliable diagnostic method for echinococcus infection in definitive hosts, e. granulosus polyoclonal antibodies (polyabs) were prepared from adult worm in rabbit. a selected poab was used for coproantigen detection in faecal samples obtained from animals naturally infected with echinococcus...