Stabilization of multimeric enzymes is one of the major challenges in biocatalysis, because dissociation of subunits can inactivate the enzyme. Catalase (CAT; EC 1.11.16) is a homotetramer containing Fe-protoporphyrin IX in its active site. CAT breaks down hydrogen peroxide into water and molecular oxygen. In this study, crosslinked enzyme aggregates of bovine liver CAT (CAT-CLEAs) were prepare...

The methodology for the preparation of cross-linked enzyme aggregates (CLEAs) and their application as highly effective, recyclable biocatalysts in a wide variety of transformations is reviewed. The method constitutes a combination of enzyme purification and immobilization into a single operation and allows for the use of relatively crude enzyme preparations. The resulting CLEAs have excellent ...

The key to obtaining an optimum performance of an enzyme is often a question of devising an effective method for its immobilization. In the present review, we describe a novel, versatile and effective methodology for enzyme immobilization as CLEAs (cross-linked enzyme aggregates). The method is exquisitely simple (involving precipitation of the enzyme from aqueous buffer followed by cross-linki...

background: timely diagnosis of leptospirosis is essential for early and effective treatment, for there are many differential diagnoses for it.. leptospiral researchers have an increasing interest in developing new serological methods with recombinant antigens to improve the leptospirosis diagnosis. several serological tests have been developed for the proper diagnosis of leptospirosis. objecti...

objectives: leptin is a hormone-like protein produced by the adipose tissue. it plays an important role in protection of host against inflammation and infection. some studies have reported changes in leptin levels in the gingival crevicular fluid (gcf), saliva and blood serum of patients with periodontal disease compared to healthy individuals. the aim of the present study was to compare the sa...

materials and methods in this descriptive study, 2988 stool samples of patients with acute gastroenteritis were collected from children’s hospitals of 5 main cities of iran. the samples were sent in frozen condition to pediatric infection research center in tehran and stored at -70°c. elisa test was performed for detection of rotavirus antigens. the mean age of study population was 1 to 5 years...

conclusions: it was concluded that the elisa is a suitable method for large scale screening of antibodies against aspergilloma. the easy techniques have shortened the required time of tests (approximately 1.30 hours for our elisa, as opposed to 2.30 hours with commercial elisa kit). this may be a substantial benefit for large numbers of samples that should be tested. materials and methods: an i...

background: nuts are one of the main consumed snacks worldwide and also have an important role among iranian's food habits. natural contamination of nuts with aflatoxin is unavoidable and causes a special challenge for nuts safety and quality. objectives: the purpose of this research was to study the aflatoxin contamination in commercially-available nuts (pistachio, walnut and peanut) in the ma...

b ackground : tuberculosis is a crucial health problem. establishing a rapid, reliable and still inexpensive diagnostic method for tuberculosis seems to be substantial in developing countries where tb has very high incidence rate . methods:   an indirect  enzyme-linked  immunosorbent  assay (elisa )   was established to detect serum antibodies against mycobacterium  tuberculosis. three kinds of...