This study was conducted on a commercial New Zealand sheep farm in order to compare the cure rates of merino sheep infected with footrot. Infected sheep were treated with either lincomycin/spectinomycin combination (Linco-Spectin soluble powder, Pfizer Animal Health) or tilmicosin (Micotil 300 Injection, ELANCO) antibiotics. A flock of 3000 mixed age merino ewes had their feet inspected by the ...

To the Editor: Several assays are available to measure human ghrelin (h-GHS), but the comparability of results among assays is unknown. As an addition to previous investigations concerning the stability of h-GHS (1 ), it was our objective to compare the two most commonly used commercial h-GHS RIAs, focusing on side-by-side determinations, recoveries, and assay imprecision. The assays were purch...

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Problem statement: Escherichia coli isolates are the most common bacterial agents of salpingitis in commercial layer hens. The objectives of this study were to determine the phylogenetic groups/subgroups and antibiotic resistance of E. coli isolates from salpingitis cases in commercial layer hens farms in southeast of Iran. Approach: One hundred twenty one E. coli isolates from oviducts of laye...

BACKGROUND The complex pathology of disease has sparked the development of novel protein expression profiling techniques that require validation in clinical settings. This study focuses on multiplexed analyses of adipocytokines and biomarkers linked to the metabolic syndrome, diabetes, and cardiovascular disease. METHODS Multiplexed immunoassays using fluorescent microspheres and the Luminex-...

The recent introduction of fluorescent bead-based technology, allowing the measurement of multiples analytes in a single 25-50 microl sample has revolutionized the study of cytokine responses. However, such multiplex approaches may compromise the ability of these assays to accurately measure actual cytokine levels. This study evaluates the performance of three commercially available multiplex c...

Recent data suggest that proinsulin is associated with cardiovascular risk factors in nondiabetic and diabetic subjects. Since most conventional insulin assays cross-react with proinsulin, it has been suggested that the associations of insulin concentrations with dyslipidemia and hypertension could actually reflect associations with proinsulin. We examined these associations by using both a con...

Santica Marcovina, Ronald R. Bowsher, Greg Miller, Myrlene Staten, Gary Myers, Samuel P. Caudill, Scott E. Campbell, Michael W. Steffes for the Insulin Standardization Work Group. 1 Northwest Lipid Metabolism and Diabetes Research Laboratory, University of Washington, Seattle, WA 2 LINCO Diagnostic Services, St. Charles MO; B2S Consulting, Beech Grove, IN 3 Department of Pathology, Virginia Com...