The purpose of this study was to evaluate the ability of spermatozoa and leukocytes in semen to produce reactive oxygen species (ROS) by using nitroblue tetrazolium (NBT) staining and to examine the association between NBT staining and levels of ROS as measured by chemiluminescence. Twenty-one infertility patients (leukocytospermia; n = 8; nonleukocytospermia, n = 13) and 9 healthy donors were ...

Dehydroabietic acid (DHA) is an analog of abietic (AA) for cardiovascular disease prevention, known to act pharmacologically against aging, inflammation, bacterial infections and cancer. The current research investigated the molecular mechanisms DHA on adhesiveness endothelial leukocytes activation NF-κB in TNF-α treated HAEC ApoE−/− mice experimental atherosclerosis. HAECs were tested toxicity...

UNLABELLED The in vitro viability, chemotaxis, and labeling stability of leukocytes labeled using (99m)Tc-exametazime stabilized with methylene blue were evaluated and compared with those obtained using nonstabilized (99m)Tc-exametazime. METHODS Two identical leukocyte populations, from 30 healthy donors, were labeled simultaneously using freshly prepared and 2-h-old stabilized (99m)Tc-examet...

The in vitro viability, chemotaxis, and labeling stability of leukocytes labeled using 99mTc-exametazime stabilized with methylene blue were evaluated and compared with those obtained using nonstabilized 99mTc-exametazime. Methods: Two identical leukocyte populations, from 30 healthy donors, were labeled simultaneously using freshly prepared and 2-h-old stabilized 99mTc-exametazime. The followi...

PURPOSE In experimental autoimmune uveitis (EAU), recent work has demonstrated that retinal damage involves oxidative stress early in uveitis, before macrophage cellular infiltration. The purpose of this study was to determine whether oxidative mitochondrial DNA damage occurs early in EAU, before leukocyte infiltration. METHODS Lewis rats were immunized with S-antigen mixed with complete Freu...

Chemokine receptor CCR2 mediates monocyte mobilization from the bone marrow (BM) and subsequent migration into target tissues. The degree to which CCR2 is differentially expressed in leukocyte subsets, and the contribution of CCR2 to these leukocyte mobilization from the BM are poorly understood. Using red fluorescence protein CCR2 reporter mice, we found heterogeneity in CCR2 expression among ...

OBJECTIVES We sought to discover which types of injuries were related to human leukocyte antigen DR expression in acute rejection and late chronic injury in renal allografts. MATERIALS AND METHODS Ninety-two recipients were separated into the early acute rejection group, the late monocyte infiltration group, and the late chronic injury group. Ten subjects with acute cellular rejection receive...

PURPOSE Leukocyte rheology may play a key role in microcirculation because leukocytes have unique properties, such as large cell volume, high cytoplasmic rigidity, and low deformability. However, only a few methods are available to study the dynamic behavior of leukocytes in retinal microcirculation. The authors developed a new method to analyze directly movements of leukocytes in the retinal v...

− 1 − Leukocyte classification on Sysmex XE series hematology analyzers involves staining leukocytes with proprietory Polymethine Dye reagents that have affinity for nucleic acids. The outputs are two-dimensional scatter diagrams of side scatter and fluorescence intensity (DIFF scattergrams) subsequent to flow cytometric analysis of the stained cells. Leukocyte classification is based on the lo...