delayed-type hypersensitivity reaction measured by skin testing, is an important tool for evaluation of cellular immune response in leishmaniasis and has been used recently as an indication of exposure to infection. skin testing in leishmania infection needs a standard reagent with high specificity, sen sitivity, and potency. in the present work, a soluble leishmanin, and three whole parasite p...

Since the early 1990s this view changed with the recognition of autochthonous cutaneous leishmaniasis. The first documented case in 1992 was a young man from Ambalantota who initially gave a history of contact with dry zone jungle but later denied this history (1). This was followed a few years later by a number of cases from different parts of the island. The second and third case reports were...

The objective of this study was to evaluate the magnitude of le ishmaniasis in the study area and to assess the role of demographic and nutritional status in the disease transmission. It was a prospective cohort study on leishmaniasis. It was conducted in 3 villages in West of Sudan. Leishmanin skin test (LST) was assessed as an epidemiological tool in a sub-clinical focus of leishmania in Suda...

INTRODUCTION The Montenegro skin test reaction and leishmaniasis lesions share fundamental characteristics of a delayed type hypersensitivity reaction. OBJECTIVES To determine whether the Montenegro skin test reaction (response to leishmanin) might approximate and thereby provide insight into the early inflammatory and immune response to Leishmania infection. MATERIALS AND METHODS We compar...

BACKGROUND The inflammatory response is prominent in the pathogenesis of dermal leishmaniasis. We hypothesized that regulatory T cells (Tregs) may be diminished in chronic dermal leishmaniasis (CDL) and contribute to healing during treatment. METHODOLOGY/PRINCIPAL FINDINGS The frequency and functional capacity of Tregs were evaluated at diagnosis and following treatment of CDL patients having...

Herein we report a 6-year-old Afghans patient referred to Department of Dermatology, St. AL-Zahra Hospital in Isfahan with ulcerative and nodular lesions disseminated over the face, arms, buttocks, pelvic girdle and legs in 2002. Direct smears of the lesions were positive for Leishman bodies. Leishmanin skin test was negative. He also had dactylitis in some digits and toes. PCR from bone biopsy...

Background: Cutaneous leishmaniasis (CL) is a self-healing disease, but due to unknown causes, a few cases of CL could get a chronic or non-healing course. These cases of chronic and recurrent leishmaniasis are defined as non-healing. Objectives: Comparison of the production of interferon-γ (IFN-γ) and Leishmanin skin test (LST) between non-healing and healed patients of CL. Patients and ...