Antibacterial activity of CNSs against Staphylococcus aureus and Escherichia coli was estimated. Higher inhibition zone 18 mm 20 were observed S. E.coli, respectively, at a concentration 2 mg/ml carbon nanosphere after 24 hrs incubation 37 ºC. In vitro cytotoxicity experiment performed on two parasite strains Leishmania donovani tropica by using MTT assay. L. revealed more sensitiv to the than ...

Visceral Leishmaniasis (VL) is one of the neglected tropical diseases in world. It becomes fatal if not diagnosed/or treated on time. The causative agent disease protozoan parasite Leishmania donovani. About 500,000 new cases VL occur annually all over world [1].

produced in man by inoculation of cultures of Leishmania tropica. Cutaneous inoculation of Leishmania donovani from spleen puncture material from a case of kala-azar, however, is reported to have ' apparently produced kala-azar in a man' but on two occasions accidental inoculation of spleen puncture material from cases of kala-azar was without any effect (Napier, 1927). Recently, Terry et al. (...

The glyoxalase system is a ubiquitous detoxification pathway that protects against cellular damage caused by highly reactive oxoaldehydes such as methylglyoxal which is mainly formed as a by-product of glycolysis. The gene encoding GLOII (glyoxalase II) has been cloned from Leishmania donovani, a protozoan parasite that causes visceral leishmaniasis. DNA sequence analysis revealed an ORF (open ...

Leishmania produce several types of mucin-like glycoproteins called proteophosphoglycans (PPGs) which exist as secretory as well as surface-bound forms in both promastigotes and amastigotes. The structure and function of PPGs have been reported to be species and stage specific as in the case of Leishmania major and Leishmania mexicana; there has been no such information available for Leishmania...

We report a case of exclusive involvement of lymph node in leishmaniasis presenting as generalized lymphadenopathy. The diagnosis of lymphatic leishmaniasis was confirmed by the presence of Leishmania donovani body in fine needle aspiration cytology, positive direct agglutination test and anti-rK39 antibodies. The bone marrow aspiration was negative for Leishmania donovani body. This is the fir...

Leishmania infantum and Leishmania donovani both pertain to the L. (L.) donovani complex. The status of certain strains is questioned in the literature and there are no reliable discriminative markers to identify them. Molecular tools are needed to (i) identify diagnostic markers and (ii) to allow a better understanding of phylogenetic relationships. We have developed a PCR based on cysteine pr...

Promastigote form of Leishmania, an intracellular pathogen, delays phagosome maturation and resides inside macrophages. But till date limited study has been done to manipulate the phagosomal machinery of macrophages to restrict Leishmania growth. Attenuated Leishmania strain exposed RAW 264.7 cells showed a respiratory burst and enhanced production of pro-inflammatory mediators. The augmentatio...