We are glad that our paper1 attracted significant attention from the journal’s readership. While we are thankful for the wise comments of Dr Jurisic about technical issues, we cannot agree with all the theses included. It is true that for our in vitro experiments the lactate dehydrogenase (LDH) microassay would have been the better choice; however, the method was not popular and we were not fam...

We have developed an automated enzymatic assay for LDH-C4, a lactate dehydrogenase (EC 1.1.1.27) isoenzyme found exclusively in spermatozoa, as a marker of human spermatogenesis. LDH-C4 activity is assayed bichromatically in the Abbott ABA-100 spectrophotometer, with 2-oxohexanoate as substrate. As many as 27 samples (2.5 microL) of human seminal plasma can be analyzed sequentially, with appare...

Activ~ties of lactic dehydrogenase (LDH), glutamate oxaloacetate transanunase (GOT) and glutamate pyruvate transaminase (GPT) were measured in blood plasma and some tissues of grey mullet Mugd auratus Risso. After determination of optimal assay conditions, cardiac sampling for LDH estimation was recommended. Tissue distribution of enzymers and their origln in plasma were described, in particula...

Lactate dehydrogenase (LDH)-immunoglobulin (Ig) complex has been reported in various diseases (1). Here we describe circulating LDH-IgG complex in a postburn patient; the complex appeared to be related to the progression of burn in this case. A 13-year-old Chinese male was admitted to our hospital with burns over 20% of his body. Some of the relevant laboratory test results and reference interv...

We incorporated anti-Parkinsonian drug, levodopa (dopa), in Zn/Al-LDH by coprecipitation method to form dopa-LDH nanocomposite. Further coating of Tween-80 on the external surfaces of dopa-LDH nanocomposite was achieved through the oxygen of C=O group of Tween-80 with the layer of dopa-LDH nanocomposite. The final product is called Tween-dopa-LDH nanocomposite. The X-ray diffraction indicates t...

BACKGROUND : Serum and sputum eosinophil cationic protein (ECP) levels are correlated with asthma disease severity. OBJECTIVE : To establish a diagnostic accuracy of ECP and lactate dehydrogenase (LDH) in serum (indirectly) and sputum (directly) as inflammatory markers in asthma. PATIENTS AND METHODS : In a cross sectional study, 76 asthmatic patients with exacerbation were enrolled in the ...

A lipopeptide extract of Bacillus amyloliquefaciens BACY1 (BLE) was found to induce cell death in human oral squamous cell carcinoma (OSCC) cell lines, SCC4 and SCC25, in this study. The results of MTT assay showed that BLE inhibited OSCC cell proliferation in a dose-dependent manner. BLE was also effective in increasing the sub-G1 phases. Furthermore, when membrane damage in SCC4 cells treated...