Introduction: To investigate the nonlinear kinetics of in vitro hepatic uptake the OATP substrate, Pitivastatin, was used as a probe. Experiments were conducted using freshly isolated rat hepatocytes, utilising the ‘oil spin’ methodology described by Hassen et al [1]. Briefly, freshly isolated rat hepatocytes were incubated with Pitvastatin (5–300μM). At 10 s, 30 s, 50 s and 70 s aliquots were ...

This paper discusses some of the recent improvements in instrumentation used for stable isotope tracer measurements in the context of measuring retinol stores, in vivo. Tracer costs, together with concerns that larger tracer doses may perturb the parameter under study, demand that ever more sensitive mass spectrometric techniques are developed. GCMS is the most widely used technique. It has hig...

A stereoselective, sensitive and rapid liquid chromatography-tandem mass spectrometry (LCMSMS) method for the determination of R-acenocoumarol and S-acenocoumarol in human plasma was developed and validated at IPRC bioanalytical labs. The procedure involved solid phase extraction of both enantiomers and their corresponding internal standard. The chromatographic separation was accomplished emplo...

Abstract This paper details the investigation of a discrete coating observed on group Egyptian panel paintings, six mummy portraits and one funerary panel, dating from first-third century CE. Six in this are encaustic, is tempera using an animal glue binder. An accretion or has been surface recesses paint layers these panels. Examination ultraviolet radiation revealed irregular visible fluoresc...

Chloramphenicol (CAP) and thiamphenicol (TAP) – analogue of CAP are widely used in the growth of animals and marine culture as they are broad spectrum antibiotics. Due to the development of resistance of microorganisms against antibiotics many countries have completely banned the use of them. A Negative Electro spray ionization mode with liquid chromatography and mass spectrometry was used to m...

BACKGROUND Although vitamin D insufficiency is prevalent in the community, only a few population-based studies have measured serum 25-hydroxy-vitamin D (25OHD) levels during pregnancy and in newborns. Maternal vitamin D deficiency has been linked to pregnancy complications, as well as hypocalcaemia and rickets in the newborn. Here, the authors report third-trimester maternal and newborn-serum 2...