BACKGROUND Vitamin D is important to health and disease. Liquid chromatography-tandem mass spectrometry (LC-MSMS) is considered the most accurate technology for quantification of serum 25-hydroxyvitamin D (25OHD) which is the best biomarker for estimating vitamin D nutritional status. METHODS Serum was mixed with acetonitrile containing hexadeuterated 25-hydroxyvitamin D(3) (d(6)-25OHD3) and ...

The objective of this study was to evaluate the analytical performance of the Abbott ARCHITECT Tacrolimus immunoassay. Proficiency panels and specimens from a population of organ transplant recipients were analyzed in 6 clinical laboratories in Europe and the United States, and the results were compared with other methods. The ARCHITECT assay requires a whole blood specimen pretreatment step wi...

Introduction The prostanoids are a family of biologically active lipids derived from the 20-carbon essential fatty acids (LCPUFA) which are involved in all aspects of the immune response including the resolution of inflammation. ω3-fatty acids, EPA DPA and DHA are anti-inflammatory, whilst the ω6fatty acid, Arachidonic acid (AA) and its metabolites: 13 (S)-HETE, TXB2, PGF2a and 6-k-PGF1a are pr...

Bu çalışma, süt ürünleri proses hatlarından izole edilen hem gram (-) de (+) biyofilm üreticisi bakterilerin QS sinyallerini göstermeyi amaçlamıştır. sinyallerinin profilini ve kimyasal bileşimini tanımlanması, mikrobiyal direnç gelişimi oluşumunun kontrolü için önemli bir faktördür. Özellikle izolatların olağan dışı davranışlarına odaklanılmıştır. İzolatların uzun zincirli AHL sinyalleri (C14-...

Markov state models (MSMs) have become the tool of choice to analyze large amounts of molecular dynamics data by approximating them as a Markov jump process between suitably predefined states. Here we investigate "Core Set MSMs," a new type of MSMs that build on metastable core sets acting as milestones for tracing the rare event kinetics. We present a thorough analysis of Core Set MSMs based o...

Abstract Leaf compounds may contribute to plant defense against Cronartium rusts. Secondary are either natural or induced in leaves. We studied the variation of leaves six alternate hosts pini and two C. ribicola that represented susceptible resistant species these Extracts from were analyzed using LC-MSMS (liquid chromatography tandem mass spectrometry) compared between same genera identify si...

Abstract We describe an analytical method for the identification, mapping and relative quantitation of glycopeptides from SARS-CoV-2 Spike protein. The may be executed using a LC-TOF mass spectrometer, requires no specialized knowledge glycan analysis exploits differential resolving power reverse phase HPLC. While this separation technique resolves peptides with high efficiency, glycans are res...