The disaccharide lactose is one of the main components of milk. During the digestion, it is hydrolysed by the enzyme ß-galactosidase into glucose and galactose. With the exception of the Caucasian populations the lactase activity decreases in most people in the age of weaning. After the ingestion of large amounts of lactose, symptoms as bloating, flatulence, abdominal pain and diarrhea can occu...

Autophagosome-lysosome fision measured by means o f electroinjected [ ‘“C] lactose While [‘“C] sucrose is an inert sequestration probe which eventually accumulates in lysosomes, [‘“c] lactose is hydrolysed immediately upon entry into the lysosome, by the lysosomal enzyme 0-galactosidase. Autophagically sequestered [“C] lactose therefore rapidly equilibrates at a low steady-state level, probably...

A multi-lumen intubation system was used to study the absorption of calcium, glucose and galactose in 13 human subjects. The intubation was placed between the duodenum abdomen and proximal jejunum and the subjects were perfused with milk and lactase-supplemented milk. Lactose disappearance over a 20 cm length of intestine was used as the index of lactase activity. The subjects were assigned to ...

crude enzyme (beta-galactosidase) extract (cee) was produced by lactobacillus ssp. bulgaricus chr hansen lb-12 and was applied in sterile milk which had been processed through ultra high temperature method (uht milk), for hydrolyzing lactose. lactosehydrolyzed milk was also produced by a pure and commercially available betagalactosidase (maxilact). optimum quantities of cee and maxilact enz...

ONE of the most interesting natural transformations in carbohydrate metabolism is that occurring in the active mammary gland during the synthesis of the galactose-containing disaccharide, lactose. Rohmann [1919] was able to obtain some evidence of the synthesis of lactose by the action of an extract of the mammary gland upon added sucrose. This investigator, on what now seems inconclusive evide...

when complexed with GTP binds any aminoacyl-tRNA formed and prevents its hydrolysis (Leder, 1973). An average of 0.97mol of tRNAIIe was charged per mol of ATP added to the reaction mix. It may be calculated from the relative reactivity of the isoleucyl-tRNA synthetase towards valine and isoleucine in the presence of tRNA and ATP (Fersht, 1977) and the relative concentrations of valine and isole...