The white rot fungus Pycnoporus cinnabarinus was characterized with respect to its set of extracellular phenoloxidases. Laccase was produced as the predominant extracellular phenoloxidase in conjunction with low amounts of an unusual peroxidase. Neither lignin peroxidase nor manganese peroxidase was detected. Laccase was produced constitutively during primary metabolism. Addition of the most ef...

Extracellular laccase was produced by Trametes versicolor IBL-04 using corn cobs as a substrate under pre-optimized culture conditions. A 64kDa laccase enzyme was purified and immobilized on calcium alginate beads using glutaraldehyde as a cross-linking reagent. Maximum enzyme immobilization efficiency (89%) was observed with 2-mm calcium-alginate beads that were developed using 4% (w/v) sodium...

A 59 kDa laccase with inhibitory activity against HIV-1 reverse transcriptase (IC(50) = 2.4 μM) was isolated from the broth of mycelial culture of the mushroom Lentinus tigrinus. The isolation procedure involved ion exchange chromatography on DEAE-cellulose and CM-cellulose, and gel filtration by fast protein liquid chromatography on Superdex 75. The laccase was adsorbed on both types of ion ex...

the aim of this study was to develop a microemulsion system as a medium for laccase-catalyzed reactions. phase behavior studies were conducted by constructing partial pseudo-ternary phase diagrams for systems comprising of cetyltrimethylammonium bromide (ctab), various organic solvents as the oil phase (i.e., hexane, cyclohexane, heptane, octane, isooctane, toluene, isopropyl myristate), two co...

The aim of the present study reported here is to examine the production, purification and characterization of the enzyme laccase from the mushroom Hypsizygus ulmarius. The culture conditions were optimized for the maximum production of enzymes with different parameters such as incubation time, carbon, nitrogen sources. The enzyme was purified by ammonium sulphate precipitation and partially cha...

The textile industries by for the most passionate user of synthetic dyes are in need of ecoefficient solutions for its coloured effluents. The decolorization and detoxification potential of white rot fungi can be harnessed. Laccase has been utilized in biological treatment of effluents containing chemical dyes. The present study is planned to study the screening and chrecterization of laccase f...

This paper presents a kinetic model of the laccase-aided chlorine dioxide bleaching of bagasse pulp. The kinetic model was based on the rate of reduction of adsorbed organic halogen (AOX). The effects of the laccase enzyme dosage, the mediator 1-hydroxybenzotriazole (HBT) dosage, and the reaction temperature on the AOX content of the bleaching effluent are discussed. Good fits were obtained for...

the rate and efficiency of decolorization of dyes likeblue ca, black b133, and corazol violet sr were tested to evaluate white rot fungal strains. trametes hirsuta and pleurotus florida showed the greatest extent ofdecolorization on nutrient salt media. maximum decolorization of 200 mg/l of blue 133 was obtained by 4days old incubated pleurotus florida followed bytrametes hirsuta after 6 days. ...

A native isolate of Pleurotus ostreatus HP-1 (Genbank Accession No. EU420068) was found to have an excellent laccase producing ability. The extracellular laccase was purified to electrophoretic homogeneity from copper sulphate induced solid-state fermentation medium by ammonium sulphate precipitation and ion-exchange chromatography. The enzyme was determined to be monomeric protein with an appa...

Abstract Micropollutants are persistent and hazardous materials in low concentrations (ng L −1 –μg ), including substances such as pharmaceuticals, personal care products industrial chemicals. The advancement of analytical chemistry has allowed for the detection micropollutants; however, an efficient economical treatment solution is yet to be installed. Fungal laccase been a successful biocatal...