This is the first report on using Catathelasma ventricosum for production of fruiting body and lignocellulosic enzymes. To improve the laccase activity and productivity of mushroom, the substrate was added with different supplements (eight aromatic compounds, Mn2+, and Cu2+). Based on the results, all these supplements can improve the laccase activity and productivity of C. ventricosum, and it ...

Various basidiomycetes and deuteromycetes, grown in liquid and solid culture media, were compared for their laccase-producing ability and for the inducing effect of soil and agricultural residues on laccase production. Laccase activity in extracts of all solid media was higher than that of the liquid media. In liquid cultures, fungal laccase activity in extracts of pea (Pisum sativum) straw-tre...

AIM To produce high laccase activities from the white-rot fungus Trametes hirsuta in an in-house air-lift bioreactor (ALB). METHODS AND RESULTS Trametes hirsuta was grown in a 6-l ALB. A fed-batch strategy with glycerol as an addition resulted in maximum laccase activity of 19,400 U l(-1), which was the highest reported from the fungus. CONCLUSION The ALB configuration with additional glyce...

A 59 kDa laccase with inhibitory activity against HIV-1 reverse transcriptase (IC(50) = 2.4 μM) was isolated from the broth of mycelial culture of the mushroom Lentinus tigrinus. The isolation procedure involved ion exchange chromatography on DEAE-cellulose and CM-cellulose, and gel filtration by fast protein liquid chromatography on Superdex 75. The laccase was adsorbed on both types of ion ex...

Transmissible hypovirulence of the chestnut blight fungus, Cryphonectria parasitica, is associated with cytoplasmic double-stranded-RNA (dsRNA) viruses. The fungal laccase has attracted interest because its activity is reduced in hypovirulent dsRNA-containing strains. A laccase cDNA clone was isolated by screening a cDNA expression library with antibodies against the purified extracellular lacc...

It has been widely reported that the white rot basidiomycete Phanerochaete chrysosporium, unlike most other white rot fungi, does not produce laccase, an enzyme implicated in lignin biodegradation. Our results showed that P. chrysosporium BKM-F1767 produces extracellular laccase in a defined culture medium containing cellulose (10 g/liter) and either 2.4 or 24 mM ammonium tartrate. Laccase acti...

Among seven carbon sources tested, glycerol and glucose favored the Cerrena unicolor laccase production (18.8-20.3 U/mL); in addition, glycerol ensured the highest manganese peroxidase (MnP) activity (2 U/mL). Substitution of glycerol with the ethanol production residue (EPR) gave the highest laccase (90.1 U/mL) activity, while the walnut pericarp provided the highest MnP activity (7.4 U/mL). S...

Azospirillum lipoferum 4T has original properties such as nonmotility, melanin synthesis, and laccase activity. Following random Tn5 mutagenesis in A. lipoferum 4T, we obtained 10 mutants which were affected in melanization and laccase activity. The class 1 mutants, with intermediate levels of laccase activity, showed some coloration; the class 2 mutants, which were completely negative for lacc...

The expression of laccase in the white rot fungus Trametes versicolor is regulated at the level of gene transcription by copper and nitrogen. We used reverse transcription-PCR to demonstrate that as the concentration of copper or nitrogen in fungal cultures was increased, an increase in laccase activity, corresponding to increased laccase gene transcription levels, was observed. In addition, we...

The laccase enzyme is the largest group of Oxidoreductase enzymes and is capable of oxidizing a wide range of organic substrates to water along with molecular oxygen resuscitation. ZnO nanoparticles are known for their specific properties such as chemical stability, high electrochemical coupling rates, and wide range of absorption of radiation as multifunctional compounds. In this study, ZnO po...