نتایج جستجو برای: l asparaginase

تعداد نتایج: 619728  

2012
Alapati Kavitha Muvva Vijayalakshmi

An enzyme-based drug, L-asparaginase, was produced by Nocardia levis MK-VL_113 isolated from laterite soils of Guntur region. Cultural parameters affecting the production of L-asparaginase by the strain were optimized. Maximal yields of L-asparaginase were recorded from 3-day-old culture grown in modified asparagine-glycerol salts broth with initial pH 7.0 at temperature 30°C. Glycerol (2%) and...

Among the two types of bacterial L-asparaginases, only type II enzymes have been used inthe treatment of acute lymphoblastic leukemia owing to their higher affinity for L-asparagine.However, current screening media used for the isolation of L-asparaginase-producingmicroorganisms do not discriminate between the two types of L-asparaginase. During anoptimization study conducted to increase L-aspa...

2006
Riccardo Riccardi John S. Holcenberg Daniel L. Glaubiger James H. Wood David G. Poplack

L-Asparaginase has been widely used for the treatment of acute lymphoblastic leukemia. Therapeutic and toxic effects in the central nervous system have been noted with systemic treatment. In order to better define the relationship between L-asparaginase administration and cerebrospinal fluid (CSF) asparagine levels, L-asparaginase and asparagine were mea sured in the CSF of rhesus monkeys follo...

Journal: :acta medica iranica 0
abdullah bani-hashem department of pediatric, school of medicine, mashhad university of medical sciences, mashhad, iran. farhad heydarian department of pediatric, school of medicine, mashhad university of medical sciences, mashhad, iran. simin hiradfar department of pathology, dr. sheikh hospital, school of medicine, mashhad university of medical sciences, mashhad, iran. hojatullah ehteshammanesh department of pediatric, school of medicine, mashhad university of medical sciences, mashhad, iran.

asparaginase is one of the most important agents in the treatment of all. however, it has some side effects including dislipidemia, hyperglycemia, coagulopathy and hepatotoxicity. we studied its side ef-fects on our patients. the effects of l-asparaginase were assessed on 25 new all patients (case group) and 25 patients with known all who had completed their treatment before. sixty two percent ...

2014
Asep A. Prihanto Mamoru Wakayama

The diverse applications of L-asparaginase have led us to explore new sources of this enzyme. Arthrospira platensis has been scarcely reported as a new candidate for L-asparaginase production. In the present study, we localized L-asparaginase in A. platensis and enhanced its production. Enzyme localization was conducted by culturing cells in SOT medium and extracting the enzymes from different ...

Journal: :Frontiers in bioscience 2014
Yingyi He Benshang Li Hui Zhang Changying Luo Shuhong Shen Jingyan Tang Jing Chen Longjun Gu

Acute Myeloid Leukemia (AML), a cancer of the myeloid line of blood cells, progresses rapidly and is typically fatal within weeks or months if left untreated. Asparaginases are a class of enzymatic anti-leukemia agents that induce apoptosis in leukemia cell lines; however, the role of L-asparaginase in the induction of apoptosis in AML cells has not been investigated. In this study, we investig...

Journal: :Indian journal of experimental biology 2015
Akansha Saxena Ramraj Upadhyay Naveen Kango

Over the recent years glutaminase free L-asparaginase has gained more importance due to better therapeutic properties for treatment of acute lymphoblastic leukemia. Actinomycetes are known for L-asparaginase activity. In the current study, 80 actinomycetes were isolated from various soil habitats by serial dilution technique. Presence of L-asparaginase was investigated in a total of 240 actinom...

2009
G. Baskar S. Renganathan

The manufacture of enzymes, like L-asparaginase with less adverse effects in the treatment of cancer is an important facet of pharmaceutical industry. Yeast and Filamentous fungi are commonly reported in scientific literature to produce L-asparaginase with less adverse effects than prokaryotic microorganisms. In the present study Latin square design was used to examine the effect of L-proline, ...

Journal: :The Journal of biological chemistry 1970
H M Suld P A Herbut

Rabbits immunized with partially purified guinea pig serum and guinea pig liver L-asparaginases produced precipitating antibodies to the homologous enzymes. Antisera to guinea pig serum L-asparaginase precipitated the guinea pig liver and agouti serum L-asparaginases but did not crossreact with Escherichia coli B L-asparaginase. Conversely, antisera to guinea pig liver L-asparaginase precipitat...

2015
Hideki Yoshida Toshihiko Imamura Akiko M. Saito Yoshihiro Takahashi So-ichi Suenobu Daiichiro Hasegawa Takao Deguchi Yoshiko Hashii Hirohide Kawasaki Mikiya Endo Hiroki Hori Nobuhiro Suzuki Yoshiyuki Kosaka Koji Kato Keiko Yumura-Yagi Junichi Hara Megumi Oda Atsushi Sato Keizo Horibe Obul Reddy Bandapalli

Although L-asparaginase related hyperglycemia is well known adverse event, it is not studied whether the profile of this adverse event is affected by intensification of L-asparaginase administration. Here, we analyzed the profile of L-asparaginase related hyperglycemia in a 1,176 patients with pediatric acute lymphoblastic leukemia treated according to the Japan Association of Childhood Leukemi...

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