OBJECTIVE We previously showed that arrest with multidose infusions of high-dose (1 mmol/L) esmolol (an ultra-short-acting beta-blocker) in oxygenated Krebs-Henseleit buffer (esmolol cardioplegia) provided complete myocardial protection after 40 minutes of normothermic (37 degrees C) global ischemia in isolated rat hearts. In this study we investigated the importance of oxygenation for protecti...

Abbreviations: Bio-LC-ATB-BMPA, 4,4-O-[2-[ 2-[2-[2-[2-[6-(biotinylamino)hexanolyl]amino]ethoxy]exoxy]ethoxy]-4-(1-azi2,2,2-trifluoroethyl)benzoyl]amino-1,3-propanediyl]bis-D-mannose; HES, HEPES, EDTA sucrose; KRH buffer, Krebs-Ringer Hepes buffer; KHB, Krebs-Henseleit bicarbonate; PBS, phosphate-buffered saline; TBS-T, Tris-buffered saline, 0.1% Tween; Carboxy-SNARF-1, 5-(and 6-)carboxy-semi-na...

T-2 toxin, a trichothecene mycotoxin, is considered to be one of the most toxic compounds that are produced by molds, particularly the Fusarium species. Fusarium species have been recognized as a great agricultural problem. They occur worldwide on a variety of plant hosts and cereal grains. The aim of this study was to investigate T-2 toxin-induced liver injury using in situ perfused rat liver....

the isolated perfused rat liver (iprl) model has been used into toxicology study of rat liver. this model provides an opportunity at evaluation of liver function in an isolated setting. studies showed that cd, in a dose-dependent manner, induced toxic effects in iprl models, and these effects were associated with aminotransferase activity and lipid peroxidation. the aim of this study was to inv...

BACKGROUND In modern biotechnology, there is a need for pausing cell lines by cold storage to adapt large-scale cell cultures to the variable demand for their products. We compared various cell culture media/solutions for cold storage of Vero-B4 kidney cells, a cell line widely used in biotechnology. RESULTS Cold storage in RPMI 1640 medium, a recommended cell culture medium for Vero-B4 cells...

Effects of divalent cations on the regulation of glucose transport and cAMP phosphodiesterase in isolated rat epididymal adipocytes were studied. EDTA (5 mM) moderately inhibited the binding of insulin to adipocytes in Krebs-Henseleit Hepes buffer. In the same buffer, A-23187 (an ionophore specific for divalent cations; 50 microM) plus EDTA (5 mM) almost completely blocked the insulin- or hydro...

We have modified the apparatus for isolated rat liver perfusion (IPRL) in order to be able to perform two perfusions simultaneously. In addition, we studied the quality and stability of livers by comparison of five different perfusates: Blood (Group A), Original Krebs Henseleit buffer (Group B), Krebs buffer with glucose (Group C) or bovine serum albumin (BSA) added, (Group D). In a last group ...

Effects of divalent cations on the regulation of glucose transport and cAMP phosphodiesterase in isolated rat epididymal adipocytes were studied. EDTA (5 mM) moderately inhibited the binding of insulin to adipocytes in Krebs-Henseleit Hepes buffer. In the same buffer, A-23187 (an ionophore specific for divalent cations; 50 p ~ ) plus EDTA (5 mM) almost completely blocked the insulinor hydrogen ...

T-2 toxin, a trichothecene mycotoxin, is considered to be one of the most toxic compounds that are produced by molds, particularly the Fusarium species. Fusarium species have been recognized as a great agricultural problem. They occur worldwide on a variety of plant hosts and cereal grains. The aim of this study was to investigate T-2 toxin-induced liver injury using in situ perfused rat liver....