The expression of intermediate filaments is sensitively reflected in cell function. To examine the involvement of keratin in a secretory function, 15 kinds of keratin (keratin-2, 3, 4, 5, 6, 7, 8, 10, 13, 14, 16, 17, 18, 19, 20) were detected immunohistochemically and immunoelectron microscopically in the rabbit duodenum. Four types of secretory cells existed in the rabbit duodenum: enteroendoc...

Sections of tissues containing lingual and extra-lingual taste buds were evaluated with monoclonal antibodies against cytokeratins. In the caudal third of the rat's tongue, keratin 20 immunoreactivity was restricted to taste buds, whereas keratins 7, 8, 18, and 19 were expressed in vallate and foliate taste buds and in cells of salivary ducts that merge with these taste epithelia. Hence, antibo...

BACKGROUND Keratin profiling studies in the developing human prostate have characterized cells thought to be stem cells and so-called intermediate cells. In a series of human prostates of various gestational ages, we extended on these studies using a comprehensive panel of keratin antibodies. MATERIALS AND METHODS Autoptic tissue from 19 fetal prostates, gestational ages between 16 and 40 wee...

Barrett's esophagus is characterized by a distinct Th2-predominant cytokine profile (IL-4) from in vivo or ex vivo evidence. The detailed role of cytokines in Barrett's esophagus, particularly whether Th2 cytokines are causative factors driving metaplastic processes, remains unknown. In this study, air-liquid interface-cultured human esophageal epithelial cells were stimulated by a Th2 cytokine...

Impaired wound healing particularly in diabetics creates a significant healthcare burden. The study aimed to evaluate the effect of keratin-butyrate fibers (FKDP +0.1%NaBu) full-thickness skin model 30 diabetic rats. Physicochemical examination showed that obtained dressing possesses heterogeneous structure and butyrate was slowly released into wound. Moreover, is nontoxic supports cell growth....

Significant progress has been made during the past decade with regard to the biochemistry and immunology of keratin filaments. The results indicate that keratin represents a family of more than 17 water-insoluble, cytoskeletal proteins,"2 that keratins are restricted to epithelia and their derivatives;d that different subsets of 210 keratins are expressed in different epithelia,1~2~7-12 that at...

Here, we report on the analysis of keratin 18 null mice. Unlike the ablation of K8, which together with K18 is expressed in embryonic and simple adult epithelia, K18 null mice are viable, fertile, and show a normal lifespan. In young K18 null mice, hepatocytes were completely devoid of keratin filaments. Nevertheless, typical desmosomes were formed and maintained. Old K18 null mice, however, de...

BACKGROUND Based on results from evaluation of tissue sections from premalignant lesions of the uterine cervix, the authors examined the hypothesis that immunostaining of Papanicolaou-stained cytologic smears with monoclonal antibodies to keratins 8 and 17 allows detection of cervical intraepithelial neoplasia (CIN) with progressive potential. They also investigated whether detection of these t...

BACKGROUND/AIM Amniotic membrane (AM) transplantation effectively expands the remaining limbal epithelial stem cells in patients with partial limbal stem cell deficiency. The authors investigated whether this action could be produced ex vivo. METHODS The outgrowth rate on AM was compared among explants derived from human limbus, peripheral cornea, and central cornea. For outgrowth of human li...

AIMS AND BACKGROUND Differential diagnosis of hepatocellular carcinoma and intrahepatic cholangiocarcinoma is sometimes difficult to accurately perform. METHODS Eight markers including cytokeratin 7 (CK7), cytokeratin 19 (CK19), MOC31, CD10, glypican 3 (GPC3), claudin 4, biglycan and high mobility group A1 (HMGA1) were immunohistochemically stained in samples from 179 surgically resected hepa...