The nucleus holds and protects the genetic information of a cell. Nuclear positioning is correlated with several important cellular processes, such as cell division, migration and differentiation, and it is essential to the development of organisms ranging from worms to positioning is mediated through a protein complex across the nuclear envelope (NE), formed by the outer nuclear membrane (ONM)...

In the fission yeast S. pombe, nuclei are actively positioned at the cell center by microtubules. Here, we show that cytoplasmic microtubules are mechanically coupled to the nuclear heterochromatin through proteins embedded in the nuclear envelope. This includes an integral outer nuclear membrane protein of the KASH family (Kms2) and two integral inner nuclear membrane proteins, the SUN-domain ...

Har vesting a new KASH crop Z hou et al. reveal that plants express diverse KASH-like proteins that perform discrete functions at the outer nuclear membrane. In animals and fungi, LINC complexes—composed of inner nuclear membrane SUN proteins and outer nuclear membrane KASH proteins— connect the nucleus to the cytoskeleton to control a variety of processes including nuclear positioning and chro...

A module $M$ is said to be coretractable if there exists a nonzero homomorphism of every nonzero factor of $M$ into $M$. We prove that all right (left) modules over a ring are coretractable if and only if the ring is Morita equivalent to a finite product of local right and left perfect rings.

In yeasts and worms, KASH (Klarsicht/ANC-1/Syne/homology) domain and SUN (Sad-1/UNC-84) domain nuclear envelope (NE) proteins play a crucial role in meiotic chromosome movement and homologue pairing. However, although the vertebrate SUN domain protein SUN1 is involved in these processes, its partner has remained identified. Based on subcellular localization screening in mouse spermatocytes, we ...

The nuclear-cytoskeleton connection influences many aspects of cellular architecture, including nuclear positioning, the stiffness of the global cytoskeleton, and mechanotransduction. Central to all of these processes is the assembly and function of conserved SUN-KASH bridges, or LINC complexes, that span the nuclear envelope. Recent studies provide details of the higher order assembly and targ...

Positioning the nucleus is essential for the formation of polarized cells, pronuclear migration, cell division, cell migration and the organization of specialized syncytia such as mammalian skeletal muscles. Proteins that are required for nuclear positioning also function during chromosome movement and pairing in meiosis. Defects in these processes lead to human diseases including laminopathies...

Klarsicht, ANC-1 and Syne homology (KASH) proteins localize to the outer nuclear membrane where they connect the nucleus to the cytoskeleton. KASH proteins interact with Sad1-UNC-84 (SUN) proteins to transfer forces across the nuclear envelope to position nuclei or move chromosomes. A new KASH protein, KDP-1, was identified in a membrane yeast two-hybrid screen of a Caenorhabditis elegans libra...

Correspondence to Gregg G. Gundersen: [email protected] Abbreviations used in this paper: ABD, actin-binding domain; CH, calponin homology; KASH, Klarsicht, ANC-1, and Syne homology; LINC, linker of nucleoskeleton and cytoskeleton; SR, spectrin repeat; SUN, Sad1 and UNC-84; TAN, transmembrane actin–associated nuclear. Introduction The linker of nucleoskeleton and cytoskeleton (LINC) complex is ...