OBJECTIVE A novel multi-drug resistance gene named as HA117 has been screened and cloned in multidrug resisitant leukemia cell lines in our previous research, but its function is still unknown. In this study, HA117 gene was investigated whether it could increase the drug resistance in chronic myelogenous myeloid leukemia cell line K562. METHODS HA117 was cloned and adenovirus vectors were con...

Background: RNA interference (RNAi) is the mechanism of gene silencing-mediated messenger RNA degradation by small interference RNA (siRNA), which becomes a powerful tool for in vivo research, especially in the areas of cancer. In this research, the potential use of an expression vector as a specific siRNA producing tool for silencing of Bcr-abl in K562 cell line has been investigated. Methods:...

Membrane currents were examined in a drug-sensitive human leukemia cell line (K562) and its multidrug-resistant cell line (K562/ADM) under the whole-cell variation of the patch electrode voltage clamp technique. Most K562 cells showed only the outward current, which was completely suppressed by internal Cs+ ions and 20 mM ethyleneglycol bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid. In ...

We have investigated the hormonal responsiveness of K562 cells using a serum-substituted in vitro clonogenic assay. Dexamethasone inhibited colony formation by the K562 cells. and the inhibitory effect could be reversed by progesterone (10 M). Fluoxymesterone caused a prominent enhancement of K562 colony growth. whereas estriol had no effect. Stimulation by triiodothyronine was maximal at iO M....

We have investigated the hormonal responsiveness of K562 cells using a serum-substituted in vitro clonogenic assay. Dexamethasone inhibited colony formation by the K562 cells, and the inhibitory effect could be reversed by progesterone (10(-6) M). Fluoxymesterone caused a prominent enhancement of K562 colony growth, whereas estriol had no effect. Stimulation by triiodothyronine was maximal at 1...

The K562/VCR cell line, exhibiting acquired multidrug resistance (MDR) with increased expression of a cell surface glycoprotein (P-glycoprotein), was isolated from human erythroleukemia K562 cells. Various compounds that induced erythroid differentiation of K562 cells were tested for their effects on growth and differentiation of these K562/VCR cells. Sodium butyrate, hemin, 1-beta-D-arabinofur...


 Purpose: To determine the effect of fresh soy milk and its compounds (coumestrol, daidzein genistein) on apoptosis in human leukemic cells peripheral blood mononuclear (PBMC).
 Methods: The apoptotic (K562, Jurkat, U937) PBMC was determined by flow cytometry. from healthy donors were isolated conducting density gradient centrifugation principle. Lymphoprep cytotoxicity evaluated 3-(...

We previously isolated aaptamine, a benzonaphthyridine alkaloid, from marine sponge Aaptos suberitoids. In this study, we investigated the anti-proliferative effect of aaptamine on chronic myeloid leukemia (CML) K562 cells. Aaptamine inhibited growth of K562 with a GI50 as 10 μM, and arrested cell cycle at G2/M phase. Western blot analysis indicated that aaptamine induced p21 expression in K562...

Ouabain has shown powerful anti-proliferation activities in various cancers, but its effect on imatinibresistant chronic myeloid leukemia and toxicity normal mice not been investigated. Cell Counting Kit-8 assay was used to detect cytotoxicity reversal of ouabain with different concentration (0.01 μM, 0.1 1.0 10 μM) drug resistance imatinib-resistant cell line (K562/G01 line). Flow cytometry th...

The aim of this study was to investigate the effect of the p15 gene combined with Bcr-abl-specific siRNA and STI571 on the proliferation, cell cycle and apoptosis of K562 chronic myeloid leukemia cells. Using the gene sequence results, we amplified the p15 gene from normal peripheral blood by RT-PCR, and constructed a p15-pcDNA3.1 vector. The K562 cell line with G418 resistance was screened, sy...