The thrips Scirtothrips dorsalis Hood (Thysanoptera: Thripidae) is an invasive pest that poses a significant economical threat to U.S. agriculture and trade. In this study, DNA sequence data and polymerase chain reaction (PCR) were utilized to develop a molecular diagnostic marker for S. dorsalis. The DNA sequence variation from the internal transcribed spacer 2 (ITS2) region of nuclear ribosom...

BACKGROUND & OBJECTIVE Anopheles fluviatilis, which ranks second among the major malarial vectors in India occurs as a complex of three morphologically identical species (species S, T and U) of which only species S is a vector. Hence, it becomes pertinent to have a method for the detection of this vector species under field conditions to map the distribution of this vector. An rDNA-ITS2-PCR ass...

Pulsatillae radix is a conventional traditional Chinese medicine (TCM) with common name Baitouweng, and has notable effects on inflammation and dysentery. Pulsatilla chinensis (Bge.) Regel is the only source plant of Baitouweng recorded in Chinese Pharmacopoeia, but its adulteration often occurs in the market that possibly affects medicinal efficacy and safety. We have established an internal t...

anopheles fluviatilis complex is known to be a vector of malaria in iran. since mosquitoes of this species cover a wide geographical range in iran, they might have evolved into different separated populations. random amplified polymorphic dna polymerase chain reaction (rapd-pcr) assay was used to differentiate geographic populations of this species. dna was extracted from individual mosquitoes ...

background : haemonchosis has a negative effect on the farming industry throughout the world, especially in the tropic and sub-tropic countries. the present study was carried out to differentiate haemonchus species from its main hosts in iran, including sheep, goat and camel.   methods: the identification took place based on the morphometrics of the spic-ules and molecular characters. two hundr...

Candida auris is an emerging multidrug-resistant yeast causing invasive health care-associated infection with high mortality worldwide. Rapid identification of C. auris is of primary importance for the implementation of public health measures to control the spread of infection. To achieve these goals, we developed and validated a TaqMan-based real-time PCR assay targeting the internal transcrib...

Serpula himantioides is a wood-decaying fungal pathogen which widespread and causes potentially serious butt rot in living trees of numerous coniferous plantation species. This study aimed to quantify S. the wood Sawara cypress (Chamaecyparis pisifera) with using real-time polymerase chain reaction (real-time PCR). Species–specific primers were designed for internal transcribed spacer 2 (ITS2) ...