نتایج جستجو برای: its2 وrdna وpcr

تعداد نتایج: 1660  

Journal: :Memorias do Instituto Oswaldo Cruz 2002
T H D A Vidigal K G Magalhães J C Kissinger R L Caldeira A J G Simpson O S Carvalho

Due to difficulties concerning morphological identification of planorbid snails of the genus Biomphalaria, and given a high variation of characters and in the organs with muscular tissue, we designed specific polymerase chain reaction (PCR) primers for Brazilian snail hosts of Schistosoma mansoni from available sequences of internal transcribed spacer 2 (ITS2) of the ribosomal RNA gene. From th...

2018
Aisha Tahir Fatma Hussain Nisar Ahmed Abdolbaset Ghorbani Amer Jamil

In pursuit of developing fast and accurate species-level molecular identification methods, we tested six DNA barcodes, namely ITS2, matK, rbcLa, ITS2+matK, ITS2+rbcLa, matK+rbcLa and ITS2+matK+rbcLa, for their capacity to identify frequently consumed but geographically isolated medicinal species of Fabaceae and Poaceae indigenous to the desert of Cholistan. Data were analysed by BLASTn sequence...

Journal: :Genome 2010
Joaquín Vierna Andrés Martínez-Lage Ana M González-Tizón

Internal transcribed spacer 1 and 2 (ITS1 and ITS2) sequences were analysed in Ensis razor shells (Mollusca: Bivalvia: Pharidae). We aimed to (1) test ITS1 and ITS2 as molecular markers at the population level in the successful alien E. directus (Conrad, 1843); (2) test these spacers at the species level in E. directus and three other Ensis species, E. siliqua (L., 1758), E. macha (Molina, 1782...

Journal: :Bioinformation 2008
Ranil Samantha Dassanayake Yasanthi Illika Nilmini Silva Gunawardene Babaranda Dhammacharige Don Nissanka Kolitha De Silva

BACKGROUND Second internal transcribed spacer (ITS2) has proven to contain useful biological information at higher taxonomic levels. OBJECTIVES This study was carried out to unravel the biological information in the ITS2 region of An. culicifacies and the internal relationships between the five species of Anopheles culicifacies. METHODOLOGY In achieving these objectives, twenty two ITS2 seq...

Journal: :Molecular ecology resources 2013
R Blaalid S Kumar R H Nilsson K Abarenkov P M Kirk H Kauserud

The nuclear ribosomal Internal Transcribed Spacer ITS region is widely used as a DNA metabarcoding marker to characterize the diversity and composition of fungal communities. In amplicon pyrosequencing studies of fungal diversity, one of the spacers ITS1 or ITS2 of the ITS region is normally used. In this methodological study we evaluate the usability of ITS1 vs. ITS2 as a DNA metabarcoding mar...

Journal: :Nucleic acids research 1999
B Michot N Joseph S Mazan J P Bachellerie

Mechanisms of ITS2 excision from pre-rRNA remain largely elusive. In mammals, at least two endonucleolytic cleavages are involved, which result in the transient accumulation of precursors to 5.8S rRNA termed 8S and 12S RNAs. We have sequenced ITS2 in four new species of the Mus genus and investigated its secondary structure using thermodynamic prediction and comparative approach. Phylogenetic e...

2016
Shangguo Feng Mengying Jiang Yujun Shi Kaili Jiao Chenjia Shen Jiangjie Lu Qicai Ying Huizhong Wang

Recently, commercial interest in Physalis species has grown worldwide due to their high nutritional value, edible fruit, and potential medicinal properties. However, many Physalis species have similar shapes and are easily confused, and consequently the phylogenetic relationships between Physalis species are poorly understood. This hinders their safe utilization and genetic resource conservatio...

2015
Shangguo Feng Yan Jiang Shang Wang Mengying Jiang Zhe Chen Qicai Ying Huizhong Wang Marcello Iriti

The over-collection and habitat destruction of natural Dendrobium populations for their commercial medicinal value has led to these plants being under severe threat of extinction. In addition, many Dendrobium plants are similarly shaped and easily confused during the absence of flowering stages. In the present study, we examined the application of the ITS2 region in barcoding and phylogenetic a...

2014
Chatchanit Arif Camille Daniels Till Bayer Eulalia Banguera-Hinestroza Adrian Barbrook Christopher J Howe Todd C LaJeunesse Christian R Voolstra

The persistence of coral reef ecosystems relies on the symbiotic relationship between scleractinian corals and intracellular, photosynthetic dinoflagellates in the genus Symbiodinium. Genetic evidence indicates that these symbionts are biologically diverse and exhibit discrete patterns of environmental and host distribution. This makes the assessment of Symbiodinium diversity critical to unders...

2017
Xiaoyue Wang Xiaochen Chen Pei Yang Lili Wang Jianping Han

Many species belonging to the genus Dendrobium are of great commercial value. However, their difficult growth conditions and high demand have caused many of these species to become endangered. Indeed, counterfeit Dendrobium products are common, especially in medicinal markets. This study aims to assess the suitability of the internal transcribed spacer 2 (ITS2) region as a marker for identifyin...

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