Of 144 patients with suspected pancreatic disease in whom a 75Se-selenomethionine scan was performed, endoscopic retrograde pancreatography (ERP) was successful in 108 (75%). The final diagnosis is known in 100 patients and has been compared with scan and ERP findings. A normal scan reliably indicated a normal pancreas, but the scan was falsely abnormal in 30%. ERP distinguished between carcino...

Complete phosphorylation mapping of protein kinases was successfully undertaken using an automated LC/MS/MS approach. This method uses the direct combination of triple quadrupole and ion trapping capabilities in a hybrid triple quadrupole linear ion trap to selectively identify and sequence phosphorylated peptides. In particular, the use of a precursor ion scan of m/z -79 in negative ion mode f...

Effect of isotope substitution from the modulation of Thermal-Lens (TL) signals in binary liquid mixtures is explored using femtosecond pump–probe mode-mismatched Z-scan TL spectroscopy. Binary solutions of water and heavy water with pure methanol at various concentrations are used for the study, where pure methanol acts as the TL marker across the different samples. The TL signal is found to b...

We reviewed all patients with a clinically infected foot ulcer attending a specialised neuropathic foot clinic. Neuropathy was confirmed by the inability to feel a 5.07 Semmes-Weinstein hair, areflexia and impaired vibration sense, as measured by a biothesiometer. Of 40 patients who attended the clinic over a two-year period, six with ischaemic ulcers were excluded. The remaining 34 had plain r...

The ability to acquire highly accurate quantitative data is an increasingly important part of any proteomics experiment, whether shotgun or top-down approaches are used. We recently developed a quantitation strategy for peptides based on neutron encoding, or NeuCode SILAC, which uses closely spaced heavy isotope-labeled amino acids and high-resolution mass spectrometry to provide quantitative d...

Measurement of isotope accumulation in an organ is often used to assess that organ's removal of blood cells labelled with the isotope. This technique is only valid if the isotope does not elute from the organ. Elution of 111In from the liver and spleen has been investigated in 14 subjects following intravenous injection of heat-damaged erythrocytes labelled with 111In. The elution rate from the...

A case is reported of a cryptococcal granuloma occurring within the lateral ventricle. The findings on angiography and brain-scanning led to a preoperative diagnosis of intraventricular meningioma. There are no previous reports of an isotope brain-scan in this condition and angiography usually shows an avascular swelling.

A 57-year-old man with splenic abscess, demonstrated by isotope scanning, ultrasonography and computerized axial tomography (CAT scan) during the course of a septicaemic illness resulting from culture-positive bacterial endocarditis, was successfully treated with antibiotics alone, resolution of the abscess being confirmed by 2 further scans. This is the second case report of successful conserv...

The findings on 99mTc dimercaptosuccinic acid (DMSA) scans were examined in 54 patients aged 3 to 33 years in whom renal scarring had been diagnosed radiologically in childhood after urinary tract infection. There was no recent history of infection. Vesicoureteric reflux had been present in 48 patients and had stopped in 23 at the time of the DMSA scan. In six of the 72 radiologically scarred k...