We successfully used the guanidine isothiocyanate method for isolation of total RNA from leaf, stem, and root tissues of the aromatic plant Origanum onites. The RNA was extracted with TRI Reagent at room temperature and was recovered by isopropanol precipitation. The isolated RNA was capable of reverse transcription. The extraction method described here does not require ultracentrifugation, and...

Well-established label in biomolecules analysis, fluorescein isothiocyanate is commercially available its two different isomers with equal fluorescence sensitivity, differing meta- vs. para-isothiocyanate position. It was found this study that the stability of depends on polarity solvents and pyridine (stabiliser) presence. The lowest has water solvent. In acidic solution, their quinoid (acid) ...

A structure-activity relationship study was carried out to identify structural features in arylalkyl and alkyl isothiocyanates that are associated with the inhibitory potency of these compounds against lung tumorigenesis induced in A/J mice by the tobacco-specific nitrosamine 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK). These features include the alkyl chain length, phenyl substitution...

The severe acute respiratory syndrome coronavirus 2, better known as COVID-19, has become a major health concern worldwide. It challenged the global healthcare sector like anything. appeared in Wuhan, China, around November 2019, had spread to almost 187 countries due its highly contagious nature. Quarantine, isolation, mask, and other precautionary measures remain sole obliging strategy declin...

A structure-activity relationship study was carried out to identify struc tural features in arylalkyl and alkyl isothiocyanates that are associated with the inhibitory potency of these compounds against lung tumorigenesis induced in A/J mice by the tobacco-specific nitrosamine 4-(methylnitrosamino)-l-(3-pyridyl)-l-butanone (NNK). These features include the alkyl chain length, phenyl substitutio...