This paper describes the evaluation of a newly developed DNA probe for Mycobacterium paratuberculosis. DNA probe PCR278 is a 278 bp fragment obtained by polymerase chain reaction (PCR) amplification of the 5'-region of IS900, an insertion element contained in the genome of M paratuberculosis. This DNA probe can specifically distinguish M paratuberculosis from a wide range of other organisms, in...

BACKGROUND Mycobacterium avium subspecies paratuberculosis (MAP) is the causative agent of Johne's disease (JD), or paratuberculosis in ruminants has been suspected to be implicated in the pathogenesis of Crohn's disease (CD) in humans with chronic inflammatory intestinal changes. As the hypothesis is now fast being recognized that MAP could possibly be the etiological agent of CD which is foun...

the efficacy of bacterial cultures and is900-specific polymerase chain reaction (pcr) was compared for the detection of mycobacterium avium subsp. paratuberculosis (map) from the mesenteric lymph nodes of goats. samples were collected from 75 goats slaughtered in ilam, in southwest of iran. tissue homogenates were inoculated onto four media. the genomic dna was extracted directly from mesenteri...

Thirty one cultures were established in MG3 medium from the intestinal tissues of 29 patients, including 18 with Crohn's disease, five with ulcerative colitis, and six non-inflammatory bowel disease controls. All cultures grew either acid fast bacilli or uncharacterized spheroplasts. Pellets from these cultures were coded and assayed blind for M paratuberculosis and M avium subsp silvaticum usi...

Mycobacterium avium subsp. paratuberculosis is a pathogen that causes chronic inflammation of the intestine in many animals, including primates, and is implicated in Crohn's disease in humans. It differs from other members of the M. avium complex in having 14-18 copies of IS900 inserted into conserved loci in its genome. In the present study, genomic DNA flanking 14 of these insertions was char...

microscopy as “field based test” was compared with is900 blood pcr for the diagnosis and estimationof prevalence of mycobacterium avium subspecies paratuberculosis (map) in domestic livestock withclinical johne’s disease (jd). of 252 animals screened, 39.3 and 13.1% were positive by faecal microscopyand blood pcr, respectively. proportional agreement between “microscopy” and blood pcr was subst...

Thirty six tissues from sheep, previously diagnosed with paratuberculosis, were tested by PCR in positive Ziehl-Neelsen staining smears of tissues, and PCR in tissues targeting IS900 specific for Mycobacterium avium subsp. paratuberculosis. DNA amplification was achieved in 33.3% Ziehl-Neelsen smears, and in 61.1% tissue samples. Combination of both techniques found 66.7% samples as positive. C...

Mycobacterium avium subsp paratuberculosis was isolated from two out of seventy samples (2.86 %) of pasteurized and ultra-pasteurized milk. The isolates were positives to IS900 PCR and showed a C17 RFLP pattern, the most prevalent in Argentina. The present study is the first report of Mycobacterium avium subsp paratuberculosis culture from pasteurized milk in Argentina.

INTRODUCTION Johne’s disease (JD) is a serious infection of animals caused by Mycobacterium avium subspecies paratuberculosis (MAP). Johne’s is not a priority in India. Therefore, information on prevalence in 465.50 million domestic ruminants have not been estimated nor realized, mainly due to lack of indigenous diagnostic kits. Diagnosis is difficult due to long incubation, absence of characte...