Enzymatic hydrolysis is an environmentally friendly technology to produce sugars from pretreated biomass. Here, we show that the new Il-11 Irpex lacteus strain can synthesize cellulases in a high quantity. The peptone and filter paper contained medium significantly enhanced activity of endo-1,4-β-D-glucanases (app. 50 IU/mL) total 9 IU/mL), whereas with sodium carboxymethyl cellulose stimulate...

The xylanase (Xyn10B) that strongly adsorbs on microcrystalline cellulose was isolated from Driselase. The Xyn10B contains a Carbohydrate-binding module family 1 (CBM1) (IrpCBMXyn10B) at N-terminus. The canonical essential aromatic residues required for cellulose binding were conserved in IrpCBMXyn10B; however, its adsorption ability was markedly higher than that typically observed for the CBM1...

BACKGROUND Irpex lacteus, a versatile lignin-degrading fungus with various extracellular enzymes, has been widely used for biological pretreatment. However, most studies have focused on the change of substrate structure after biological pretreatment, and the effect of these changes on the enzymatic hydrolysis, but the effect of byproducts from biological pretreatment process on subsequent enzym...

Olive mill wastewater (OMW) constitutes a major cause of environmental pollution in olive-oil producing regions. Sixty wood-rot macrofungi assigned in 43 species were evaluated for their efficacy to colonize solidified OMW media at initially established optimal growth temperatures. Subsequently eight strains of the following species were qualified: Abortiporus biennis, Ganoderma carnosum, Hapal...

The expediency of using unique SSR DNA-markers Schizophyllum commune for population genetic assays in various fungal species has been demonstrated. In Auricularia auricula-judae and Irpex lacteus, we observed formation heterogeneous amplicons mostly up to 500 bp length that ensured their high resolution facilitated data analysis. established sets molecular markers are efficient DNA-fingerprinti...