The expression of invertases was analyzed in vegetative organs of well-watered and water-stressed maize (Zea mays) plants. Early changes in sucrose metabolism and in acid soluble invertase expression were observed in vegetative sink and source organs under mild water stress. The organ-specific induction of acid invertase activity was correlated with an increase in the Ivr2 gene transcripts and ...

Yeast invertase forms a homo-octamer of core glycosylated subunits during assembly in the lumen of the endoplasmic reticulum. This form has been purified from mutant cells (sec18) in which transport of secreted proteins from the endoplasmic reticulum is blocked. No heterologous protein subunits are found in the purified material. Analysis of invertase derived from wild type cells or from mutant...

Invertase plays multiple pivotal roles in plant development. Thus, its activity must be tightly regulated in vivo. Emerging evidence suggests that a group of small proteins that inhibit invertase activity in vitro appears to exist in a wide variety of plants. However, little is known regarding their roles in planta. Here, we examined the function of INVINH1, a putative invertase inhibitor, in t...

The yeast SUC2 gene is a structural gene for both the secreted and intracellular forms of invertase. We have determined the nucleotide sequence of the coding region and the 5' and 3' flanking regions. The coding regions for the signal peptide-containing precursor to secreted invertase and for the intracellular invertase begin at different initiation codons within the SUC2 gene but share the sam...

Invertase production by wild cultures of Saccharomyces cerevisiae isolated from dates available in local market is reported. Five hyperproducing yeast strains (>100 fold higher invertase activity) were kinetically analyzed for invertase production. Saccharomyces cerevisiae strain GCAII was found to be a better invertase yielding strain among all other isolates. The values of Qp and Yp/s for GCA...

The sharing of secreted invertase by yeast cells is a well-established laboratory model for cooperation, but the only evidence that such cooperation occurs in nature is that the SUC loci, which encode invertase, vary in number and functionality. Genotypes that do not produce invertase can act as 'cheats' in laboratory experiments, growing on the glucose that is released when invertase producers...

In an investigation of the minor sugars of honey at this laboratory, examination by paper chromatography indicated that several oligosaccharides are present. These have not yet been identified. Since honey is the product of the reaction of honeybee (1) invertase with nectar sugars [sucrose, glucose, and fructose (2)], a comparison is being made of the sugars of honey with the oligosaccharides p...

Yeast secretory mutants sec53 and sec59 define a posttranslational stage in the penetration of glycoprotein precursors into the endoplasmic reticulum (ER). In the previous report we showed that at the restrictive temperature (37 degrees C) these mutants accumulate enzymatically inactive and incompletely glycosylated forms of the secretory enzyme invertase and the vacuolar enzyme carboxypeptidas...

effect of different co2 concentrations on sucrose metabolizing enzymes and on carbohydrate metabolism was studied for eight blackgram (vigna mungo l. hepper) genotypes grown in open top chambers under ambient (380 µmol mol-1) vs. elevated co2 (550 and 700 µmol mol-1) levels. the higher acid invertase activity over neutral invertase indicated the major role of acid invertase in sucrose breakdown...