in order to assess drought tolerance of mycorrhizal petunia plants (petunia hybrida cv. mix), a greenhouse experiment was carried out with two different mycorrhizae species (glomus mosseae and glomus intraradices) applied singly or in combination at two phosphorous (p) levels (0 and 100 mg kg-1 dry soil) and three irrigation regimes (100% field capacity (fc) as control, 75% fc producing moderat...

Because of the high genetic variability of the nuclear-encoded ribosomal RNA genes within single spores of arbuscular mycorrhizal fungi (AMF, Glomeromycota), new alternative marker genes are needed to better resolve closely related taxa. Based on published nucleotide sequences of the mitochondrial large ribosomal subunit (mt-LSU), new fungal-specific primers were constructed and successfully us...

To explore the molecular mechanisms that prevail during the establishment of the arbuscular mycorrhiza symbiosis involving the genus Glomus, we transcriptionally analysed spores of Glomus intraradices BE3 during early hyphal growth. Among 458 transcripts initially identified as being expressed at presymbiotic stages, 20% of sequences had homology to previously characterized eukaryotic genes, 30...

This experiment aims at establishing the ability of the endomycorrhizal fungus Glomus intraradices to suppress the plant pathogen Pythium ultimum in cherry tomato. A study published in Canadian Journal of Plant Pathology in 1994 showed that Glomus intraradices suppresses Pythium ultimum in Tagetes patula (french marigold) (St-Arnaud, Fortin, Caron, & Hamel, 1994). Pythium ultimum and other Pyth...

The study evaluated the response of alfalfa (Medicago sativa L) to arbuscular mycorrhizal fungi (AM) species Glomus intraradices and Sinorhizobium meliloti, strain 1021 regarding the dry biomass accumulation, mycorrhizal fungi colonization, nodulation and nitrogen fixation activity. Alfalfa plants were grown in a glasshouse until the flowering stage (58 days), in 4 kg plastic pots using leached...

Attachment of certain bacteria to living arbuscular mycorrhizal fungal extraradical hyphae may be an important prerequisite for interactions between these microorganisms, with implications for nutrient supply and plant health. The attachment of five different strains of gfp-tagged soil bacteria (Paenibacillus brasilensis PB177 (pnf8), Bacillus cereus VA1 (pnf8), Pseudomonas fluorescens SBW25<gf...

Previous studies have found that some phosphate (Pi) starvation inducible transporter genes are downregulated and arbuscular mycorrhizal (AM) inducible Pi transporter genes are upregulated in maize roots associated with the fungus Glomus intraradices. However, little is known about the functional diversity of different AM fungal species in influencing the expression of Pi transporters in maize ...

The genome size, complexity, and ploidy of the arbuscular mycorrhizal fungus (AMF) Glomus intraradices was determined using flow cytometry, reassociation kinetics, and genomic reconstruction. Nuclei of G. intraradices from in vitro culture, were analyzed by flow cytometry. The estimated average length of DNA per nucleus was 14.07+/-3.52 Mb. Reassociation kinetics on G. intraradices DNA indicate...

An interaction study was performed with mycorrhizal plants of the grapevine rootstock Richter 110 (Vitis berlandieri Planch × Vitis rupestris Scheele) and the root pathogenic fungus Armillaria mellea (Vahl:Fr.) P. Kumm using an autotrophic in vitro culture system. Micropropagated plantlets were transferred to Petri plates with MSR medium lacking sugar and vitamins. Inocula of Glomus intraradice...

Lipids are the major form of carbon storage in arbuscular-mycorrhizal fungi. We studied fatty acid synthesis by Glomus intraradices and Gigaspora rosea. [(14)C]Acetate and [(14)C]sucrose were incorporated into a synthetic culture medium to test fatty acid synthetic ability in germinating spores (G. intraradices and G. rosea), mycorrhized carrot roots, and extraradical fungal mycelium (G. intrar...